We narrowed to 254,303 results for: addgene

1. pcDNA3.2 mir-1-1 reporter mir16-1.3

   Plasmid

   #46682

   Purpose

   Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   mir16-1.3 (MIR16-1 Human, Synthetic)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   see sequence and publication

   Promoter

   CMV

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.2 mir-1-1 reporter mir16-1.1

   Plasmid

   #46680

   Purpose

   Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   mir16-1.1 (MIR16-1 Human, Synthetic)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   see sequence and publication

   Promoter

   CMV

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pONL-C1_fibrillarin

   Plasmid

   #65710

   Purpose

   Expresses ONL-fibrillarin in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   fibrillarin (FBL Human)
   

   Use

   Luciferase

   Tags

   ONL

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pYNL-C1_fibrillarin

   Plasmid

   #65708

   Purpose

   Expresses YNL-fibrillarin in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   fibrillarin (FBL Human)
   

   Use

   Luciferase

   Tags

   YNL

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMKO.1-puro/hFOXH1 shRNA6

   Plasmid

   #59310

   Purpose

   Knockdown of human FOXH1

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

   Insert

   hFOXH1 shRNA (FOXH1 Human)
   

   Use

   RNAi and Retroviral

   Promoter

   hU6

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

6. SL542

   Plasmid

   #49951

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD RH-8 (RHOXF2 Human)
   

   Use

   TALEN

   Promoter

   EF1a

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

7. SL544

   Plasmid

   #49970

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD RH-10 (RHOXF2 Human)
   

   Use

   TALEN

   Promoter

   EF1a

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

8. SL545

   Plasmid

   #49953

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD RH-11 (RHOXF2 Human)
   

   Use

   TALEN

   Promoter

   EF1a

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

9. SL543

   Plasmid

   #49952

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD RH-9 (RHOXF2 Human)
   

   Use

   TALEN

   Promoter

   EF1a

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

10. SL541

    Plasmid

    #49950

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-7 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

11. SL540

    Plasmid

    #49949

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-6 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

12. FNE2-A72

    Plasmid

    #44608

    Depositor

    Robert Oshima

    Article

    Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

    Insert

    Ets2-A72 (Ets2 Mouse)
    

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    THR-72 converted to Alanine

    Promoter

    CMV

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

13. GoldyTALEN-ppp1cab TAL2

    Plasmid

    #41836

    Depositor

    Jeffrey Essner

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    protein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)
    

    Use

    Cloning vector

    Available Since

    Feb. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. GoldyTALEN-moesina TAL1

    Plasmid

    #41831

    Depositor

    Jeffrey Essner

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    moesin a (msna Zebrafish)
    

    Use

    Cloning vector

    Available Since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GoldyTALEN-moesina TAL2

    Plasmid

    #41832

    Depositor

    Jeffrey Essner

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    moesin a (msna Zebrafish)
    

    Use

    Cloning vector

    Available Since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCaShs-Tum

    Plasmid

    #37303

    Depositor

    Norbert Perrimon

    Article

    Harrison et al EMBO J. 1995 Jun 15;14(12):2857-65.

    Insert

    hopTum-1 (hop Fly)
    

    Expression

    Insect

    Mutation

    Tul-l mutation is a G to A transversion at nucleo…

    Promoter

    Hsp70

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

    Plasmid

    #115141

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

    Depositor

    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized Orf2mor anti-CRISPR protein
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEN_TTmiRc2_3xflag_CHEK2

    Plasmid

    #136526

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    CHEK2 (CHEK2 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3x FLAG

    Availability

    Academic Institutions and Nonprofits only

    

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19. tet-pLKO.1_puro_shJUND #2

    Plasmid

    #136583

    Purpose

    Expresses an inducible short hairpin targeting human JUND sequence

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    shJUNDv2 (JUND Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIVTRup

    Plasmid

    #101362

    Purpose

    Highly efficient in vitro transcription. Empty backbone.

    Depositor

    Ángel Raya

    Insert

    5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
    

    Use

    For cloning a gene for in vitro transcription pur…

    Promoter

    T7

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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