Addgene

We narrowed to 3,118 results for: ER

Showing: 821 - 840 of 3118 results

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PEP114E

Plasmid

#97400

Purpose

binary construct of ER-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Use

Tags

ER target

Expression

Plant

Mutation

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Cenp-B INCENP mCherry

Plasmid

#45235

Purpose

Target INCENP to centromeres via full length CENP-B

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B, INCENP-Δcen, mCherry

Use

Tags

full length CENP-B and mCherry

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV2-CARP1

Plasmid

#16014

Depositor

Wafik El-Deiry

Article

McDonald et al Proc Natl Acad Sci U S A. 2004 Apr 20. 1

Insert

CARP1 (RNF34 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pAc-sgRNA-Cas9.scr_2

Plasmid

#190704

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_1.2μ-pcDNA3

Plasmid

#111926

Purpose

Cyan color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_1.2μ

Use

Tags

Expression

Mammalian

Mutation

E67D, E104D, D133E at CaM

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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TRAPa-3xFLAG-pCDNA

Plasmid

#201963

Purpose

Mammalian expression of Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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OeNL(Ca2+)_18μ-pcDNA3

Plasmid

#111928

Purpose

Orange color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

OeNL(Ca2+)_18μ

Use

Tags

Expression

Mammalian

Mutation

E67D, E104D, D133E at CaM

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-YOD1 C160S

Plasmid

#49785

Purpose

Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

Depositor

Christian Schlieker

Article

Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

Insert

YOD1 (YOD1 Human)

Use

Tags

Flag-tag

Expression

Mutation

C160S

Promoter

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only
CeNL(Ca2+)_19μ-pcDNA3

Plasmid

#111927

Purpose

Cyan color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_19μ

Use

Tags

Expression

Mammalian

Mutation

E67D, F92W, E104D, D133E at CaM

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PsopB-GFPLVA

Plasmid

#23341

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology. 2009 Dec 24. ():.

Insert

GFP(LVA)

Use

Tags

GFP(LVA)

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pAcUW51-gE2

Plasmid

#11621

Depositor

Pamela Bjorkman

Article

Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

Insert

HSV-1 glycoprotein E

Use

Tags

His

Expression

Insect

Mutation

deleted amino acids 391-550

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pBMN-flag beta-catenin K19R/K49R

Plasmid

#44751

Depositor

Eric Fearon

Article

Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

Insert

beta-catenin

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K19R/K49R

Promoter

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pMPMA3dPlac-PpipB-GFPLVA

Plasmid

#23344

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology. 2009 Dec 24. ():.

Insert

GFP(LVA)

Use

Tags

GFP(LVA)

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B IRES GFP

Plasmid

#133817

Purpose

Encodes the C2B domain of synaptotagmin 1 for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

Promoter

hSyn (human synapsin I promoter)

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBMN-flag beta-catenin K49R

Plasmid

#44750

Depositor

Eric Fearon

Article

Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

Insert

beta-catenin

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K49R

Promoter

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pBMN-flag beta-catenin K19R

Plasmid

#44749

Depositor

Eric Fearon

Article

Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

Insert

beta-catenin

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K19R

Promoter

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pF(UG) hSyn SYP-SELF

Plasmid

#158770

Purpose

Expression of synaptophysin 1 variant with self-cleaving c-terminal tail.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

Synaptophysin 1-NS3/4a (Syt1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Flexible linkers, ADVVCC/QMSY cleavage site

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A IRES GFP

Plasmid

#133818

Purpose

Encodes the C2A domain of synaptotagmin 1 for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

Promoter

hSyn (human synapsin I promoter)

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-CNPshRNA2

Plasmid

#126612

Purpose

Expresses shRNA against human CNP under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

CNP ShRNA-2

Use

RNAi

Tags

Expression

Mutation

Promoter

Mouse U6 promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pERB161

Plasmid

#58286

Purpose

const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-584 (MID)

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-GFP-FKBPx3
inducible FRB-mCh-Mad1^485-584
FKBP-targeted miRNA

Use

Tags

GFP and mCherry

Expression

Mammalian

Mutation

amino acids 485-584 of Mad1

Promoter

Available since

Feb. 19, 2015

Availability

Academic Institutions and Nonprofits only

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