... FP fusion with a resident endoplasmic reticulum (ER) protein instead localizes to the cytoplasm? Or more...absolute position requirements. For example, a KDEL ER retrieval sequence only functions at the extreme ...potential to interfere with the function of resident ER chaperones and may impact cellular function or possibly...complex-localized fusions from properly exiting the ER and fluorescing (see Figure 2) [5].  When using FPs... pathway, a dark pool that is mislocalized to the ER can be detected with immunofluorescence. This population...through the secretory pathway, and retained in the ER. FPs have been undeniably powerful and successful...

... the ER lumen reaches the sub-millimolar range, much higher than in the cytosol. Thus, for the ER you ...measuring the events-of-interest. Sensors suitable for ER measurements include: CEPIA (additional versions ... to simultaneously measure calcium changes in the ER, cytosol and mitochondria), the Cameleon-based FRET...

...fluorescent protein to the Endoplasmic Reticulum (ER). If homodimerization occurs (most likely in an antiparallel...used as positive and negative controls for proper ER structure. Based on these data, we conclude that ...tagging. On the other hand, mScarlet-I shows correct ER labeling, which is good evidence that mScarlet-I ...

... lab's SARS-CoV-2 sybodies! References Geertsma ER (2013) FX Cloning: A Versatile High-Throughput Cloning...Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA (2020) Generation of synthetic nanobodies...Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA (2018) Synthetic single domain...

...communication specialist (you'll know me by my Tweeting — er, posting!) and our favorite content strategist, Jo...

...S, Pelham HRB (1986) An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein...A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016...

... multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New viral...

...ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain (LDB) to the C-terminus of...ligand Tamoxifen is added. Tamoxifen then binds to the ER-LDB domains of the fusions allowing Cre/FLP translocate...

...Reticulum ER retention signal E2-Crimson Benjamin Glick 36204 pmTurquoise2-ER Endoplasmic Reticulum ER retention...Gadella 137804 ER-mNeonGreen Endoplasmic Reticulum KDEL sequence mNeonGreen Dorus Gadella 137805 ER-mScarletI...Endoplasmic Reticulum ER retention signal moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention signal...Erik Snapp 49150 BFP-KDEL Endoplasmic Reticulum BiP ER signal sequence BFP Gia Voeltz 61807 Rtn4a-GFP Endoplasmic...

...the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes. Examples include...

... Figure 2: Two-color super-resolution imaging of ER and mitochondria reveals dynamic organelle interactions...interactions. a) Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta) and outer...

...ESR2 Plasmids ESR2, ER-BETA, ESR-BETA,ESRB, ESTRB, Erb, NR3A2 estrogen receptor 2 (ER beta) ESRRA Plasmids...

...Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules ...of secretory proteins, from their synthesis in the ER to their arrival in their target compartment (e.g...stably localized in the donor compartment (e.g. the ER) and a reporter protein of interest (Figure A). Franck...and interactions between the endoplasmic reticulum (ER), mitochondria, cytoskeleton, and endosomes. These...

...and cytosolic RCaMP1h calcium sensors, ER-targeted CEPIA1er ER-mitochondria tethering by PDZD8 regulates...2023.07.18.549527 Eric Schreiter Calcium GCEPIA1-SNAP ER for sensing ER calcium A genetically encoded ratiometric ...Calcium Cytosolic GCaMP6F, mitochondria Mitycam, or ER targeted GCaMPer Multiplexed Optical Sensors in Arrayed...live cells. eLife. 2018 Jul 3;7. Jin Zhang Calcium ER or mitochondria-targeted CEPIA calcium sensors (green...Merkx Zinc eZinCh-2 Zn2+ FRET sensor, cytosolic or ER-targeted eZinCh-2: A Versatile, Genetically Encoded...Cyan-Yellow Zinc FRET sensors ZapCY1 and ZapCY2, Golgi or ER targeted Measuring steady-state and dynamic endoplasmic...fluorescent protein sensor reveals surprising stability of ER thiol redox. J Cell Biol. 2013 Apr 15;201(2):337-...

...Cre is tamoxifen. In tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain...

... phosphatase. Normally PHO8 is synthesized at the ER, delivered to the vacuole via the secretory pathway...

...A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS One, 2014. 9(6): p. ...

...to label proteins moving to and from the nucleus, ER, mitochondria, stress granules, and more. For added...

...References 1. Snapp, E.L., et al., Formation of stacked ER cisternae by low affinity protein interactions. J...

...also provided CIB constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors ...