... FP fusion with a resident endoplasmic reticulum (ER) protein instead localizes to the cytoplasm? Or more...absolute position requirements. For example, a KDEL ER retrieval sequence only functions at the extreme ...potential to interfere with the function of resident ER chaperones and may impact cellular function or possibly...complex-localized fusions from properly exiting the ER and fluorescing (see Figure 2) [5].  When using FPs... pathway, a dark pool that is mislocalized to the ER can be detected with immunofluorescence. This population...through the secretory pathway, and retained in the ER. FPs have been undeniably powerful and successful...

... the ER lumen reaches the sub-millimolar range, much higher than in the cytosol. Thus, for the ER you ...measuring the events-of-interest. Sensors suitable for ER measurements include: CEPIA (additional versions ... to simultaneously measure calcium changes in the ER, cytosol and mitochondria), the Cameleon-based FRET...

...fluorescent protein to the Endoplasmic Reticulum (ER). If homodimerization occurs (most likely in an antiparallel...used as positive and negative controls for proper ER structure. Based on these data, we conclude that ...tagging. On the other hand, mScarlet-I shows correct ER labeling, which is good evidence that mScarlet-I ...

... lab's SARS-CoV-2 sybodies! References Geertsma ER (2013) FX Cloning: A Versatile High-Throughput Cloning...Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA (2020) Generation of synthetic nanobodies...Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA (2018) Synthetic single domain...

...communication specialist (you'll know me by my Tweeting — er, posting!) and our favorite content strategist, Jo...

...S, Pelham HRB (1986) An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein...A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016...

... multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New viral...

...ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain (LDB) to the C-terminus of...ligand Tamoxifen is added. Tamoxifen then binds to the ER-LDB domains of the fusions allowing Cre/FLP translocate...

...the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes. Examples include...

... Figure 2: Two-color super-resolution imaging of ER and mitochondria reveals dynamic organelle interactions...interactions. a) Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta) and outer...

...Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules ...of secretory proteins, from their synthesis in the ER to their arrival in their target compartment (e.g...stably localized in the donor compartment (e.g. the ER) and a reporter protein of interest (Figure A). Franck...and interactions between the endoplasmic reticulum (ER), mitochondria, cytoskeleton, and endosomes. These...

... phosphatase. Normally PHO8 is synthesized at the ER, delivered to the vacuole via the secretory pathway...

...Cre is tamoxifen. In tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain...

...A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS One, 2014. 9(6): p. ...

...to label proteins moving to and from the nucleus, ER, mitochondria, stress granules, and more. For added...

...References 1. Snapp, E.L., et al., Formation of stacked ER cisternae by low affinity protein interactions. J...

...also provided CIB constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors ...

...how you can make CRISPR mouse model generation easi-er! Generating mice before CRISPR The process of generating...

...results, so you only need to pick your favorite (...er, whichever one uses assumptions that match your data...

... previously unsuspected genes with known roles in ER-associated degradation (ERAD) that positively regulate...