We narrowed to 32,439 results for: Eng;

1. pCTCON2-BXG-2TAG

   Plasmid

   #158138

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment
   

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the first serine residue…

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6_Ptrc_BsMdh_BmHps_BmPhi

   Plasmid

   #129104

   Purpose

   Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

   Depositor

   Eleftherios Terry Papoutsakis

   Article

   Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

   Insert

   BsMDH BmHPS BmPHI
   

   Expression

   Bacterial

   Available Since

   Nov. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pETM6-Pc4CL-PhCHS-CmCHI

   Plasmid

   #73397

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   Pc4CL
   PhCHS
   CmCHI
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFNC-2

   Plasmid

   #227667

   Purpose

   Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. KmR, easily curable via sucrose counterselection.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   KmR
   

   Expression

   Bacterial

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCIFR-5

   Plasmid

   #233294

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Promoter

   p14G-BCD2

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIFR-8

   Plasmid

   #227666

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Am) used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   AmR
   

   Expression

   Bacterial

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIFR-6

   Plasmid

   #227665

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Gm) used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Expression

   Bacterial

   Promoter

   p14G-BCD2

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIFR-4

   Plasmid

   #227664

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Sm) used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Expression

   Bacterial

   Promoter

   p14G-BCD2

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIFR-2

   Plasmid

   #227663

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Km) used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Expression

   Bacterial

   Promoter

   p14G-BCD2

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFNC-8

    Plasmid

    #227670

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. AmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    AmR
    

    Expression

    Bacterial

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFNC-4

    Plasmid

    #227668

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. SmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    SmR
    

    Expression

    Bacterial

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. SBC015898

    Plasmid

    #226285

    Purpose

    Expresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.

    Depositor

    Rainer Breitling

    Article

    Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

    Inserts

    Coniferyl alcohol acyltransferase
    Eugenol synthase 1
    Eugenol O-methyltransferase
    

    Expression

    Bacterial

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. iNicSnFR12

    Plasmid

    #227142

    Purpose

    Genetically encoded fluorescent biosensor for nicotine detection

    Depositor

    Henry Lester

    Article

    Haloi et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Insert

    iNicSnFR12
    

    Tags

    6xHis-HA and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTC3cIfm

    Plasmid

    #193778

    Purpose

    Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    Frame-shifted CI
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PLTetO-1

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTS2341_Tier3(SB)-Puro

    Plasmid

    #169639

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven PuroR expression
    

    Expression

    Mammalian

    Promoter

    PRPBSA

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVH616

    Plasmid

    #169605

    Purpose

    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Expression

    Mammalian

    Promoter

    PCREm4 / PPGK

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169592

    Purpose

    Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    phloretin-controlled SEAP and iRFP expression
    

    Expression

    Mammalian

    Promoter

    TtgO2-PCMVmin-2

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV

    Plasmid

    #169589

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Expression

    Mammalian

    Promoter

    TetO7-PCMVmin-2

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTS2393-Tier1-PhCMV-p2a_fastFT

    Plasmid

    #169530

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused fluorescent timer, fast maturation time
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVH1001-Tier2(ColE1 ori)

    Plasmid

    #169599

    Purpose

    Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and ColE1 ori).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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