Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 832 results for: Neb

Clear keywords
Showing: 821 - 832 of 832 results

  1. pChlr-7NQ

    Plasmid
    #52169
    Purpose
    Encodes module 7 with amino acids 12N-16Q for recognition of nt 2U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12S to 12N and 16E to 16Q in module 7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pChlr-7SYR

    Plasmid
    #52170
    Purpose
    Encodes module 7 with amino acids 12S-13Y-16R for recognition of nt 2C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 13N to 13Y and 16E to 16R in module 7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pChlr-4SR

    Plasmid
    #52154
    Purpose
    Encodes module 4 with amino acids 12S-16R for recognition of nt 5C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 4
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pChlr-4CYQ

    Plasmid
    #52155
    Purpose
    Encodes module 4 with amino acids 12C-13Y-16Q for recognition of nt 5A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C and 13H to 13Y in Module 4
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pChlr-5SE

    Plasmid
    #52160
    Purpose
    Encodes module 5 with amino acids 12S-16E for recognition of nt 4G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16E in Module 5
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pChlr-6SR

    Plasmid
    #52166
    Purpose
    Encodes module 6 with amino acids 12S-16R for recognition of nt 3C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 6
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pCas9_sgRNA_0

    Plasmid
    #70763
    Purpose
    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10
    Inserts
    U6 promoter
    cas9
    tnos terminator
    Use
    Self-replicating in ustilago maydis, conferring c…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U6 from Ustilago maydis and synthetic otef promot…
    Available Since
    Nov. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    70763-plasmid-map-sequence-id-240922

    Enlarge

  10. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110629-plasmid-map-sequence-id-210665

    Enlarge

  11. POMBOX MoClo YTK

    Plasmid Kit
    #1000000251
    Purpose
    Seamless and efficient assembly of single gene and multigene plasmids for genome integration in Schizosaccharomyces pombe.
    Depositor
    Tomáš Pluskal
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only

  12. PUF Assembly Kit

    Plasmid Kit
    #1000000051
    Purpose
    Assembly of Pumilio and FBF (PUF) homology domain based RNA-binding proteins for recognition of RNA targets.
    Depositor
    Huimin Zhao
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Bacterial Expression, Mammalian Expression
    Editing Type
    PUF
    Cloning Type
    Golden Gate
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 821 - 832 of 832 results