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pcDNA3-CARMA2sh-E138A

Plasmid

#97068

Purpose

Expresses Psoriasis-Linked mutant CARD14/CARMA2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

HA

Expression

Mammalian

Mutation

changed Glutamic Acid 138 to Alanine

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

Plasmid

#129028

Purpose

inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-hudTET1CD, SgRNA cloning site

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A), catalytic domain huTET1 inact…

Promoter

CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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MARK2 gRNA (BRDN0001145542)

Plasmid

#77591

Purpose

3rd generation lentiviral gRNA plasmid targeting human MARK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MARK2 (MARK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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COPE_pLX307

Plasmid

#98327

Purpose

Lentiviral expression of COPE

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

COPE (COPE Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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KSR1 gRNA (BRDN0001146869)

Plasmid

#77858

Purpose

3rd generation lentiviral gRNA plasmid targeting human KSR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KSR1 (KSR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KSR1 gRNA (BRDN0001146294)

Plasmid

#77859

Purpose

3rd generation lentiviral gRNA plasmid targeting human KSR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KSR1 (KSR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KSR1 gRNA (BRDN0001147076)

Plasmid

#77860

Purpose

3rd generation lentiviral gRNA plasmid targeting human KSR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KSR1 (KSR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KSR2 gRNA (BRDN0001145237)

Plasmid

#76427

Purpose

3rd generation lentiviral gRNA plasmid targeting human KSR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KSR2 (KSR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KSR2 gRNA (BRDN0001147424)

Plasmid

#76425

Purpose

3rd generation lentiviral gRNA plasmid targeting human KSR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KSR2 (KSR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KSR2 gRNA (BRDN0001148172)

Plasmid

#76426

Purpose

3rd generation lentiviral gRNA plasmid targeting human KSR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KSR2 (KSR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CHUK gRNA (BRDN0001149372)

Plasmid

#77033

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHUK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CHUK (CHUK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CHUK gRNA (BRDN0001149269)

Plasmid

#77034

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHUK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CHUK (CHUK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BRAF gRNA (BRDN0001146266)

Plasmid

#76480

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRAF

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRAF (BRAF Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHA2 gRNA (BRDN0001147157)

Plasmid

#76368

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHA2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHA2 (EPHA2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CSNK1A1 gRNA (BRDN0001145896)

Plasmid

#76190

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1A1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1A1 (CSNK1A1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EIF2AK2 gRNA (BRDN0001148668)

Plasmid

#75639

Purpose

3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EIF2AK2 (EIF2AK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MARK3 gRNA (BRDN0001145035)

Plasmid

#77716

Purpose

3rd generation lentiviral gRNA plasmid targeting human MARK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MARK3 (MARK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AKT1 gRNA (BRDN0001149266)

Plasmid

#75501

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT1 (AKT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK14 gRNA (BRDN0001147002)

Plasmid

#76638

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK14

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK14 (CDK14 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK14 gRNA (BRDN0001146521)

Plasmid

#76639

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK14

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK14 (CDK14 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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