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pT.cruzi-beta-tubulin_6xHis

Plasmid

#256086

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Beta tubulin, putative

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-pa-u6ac-Vamp8-guides

Plasmid

#254764

Purpose

Vamp8 gRNA for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Publication

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Vamp8 (vamp8 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-DNAJC13(351t)

Plasmid

#246666

Purpose

Mammalian expression of truncated, eGFP tagged DNAJC13 (PH-like domain only)

Depositor

Braden Lobingier

Publication

Adoff et al Mol Biol Cell. 2025 Sep 1;36(9):ar114. d

Insert

DNAJC13 (DNAJC13 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Deletion of residues 352 to 2243

Promoter

CMV

Available since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mGL-bGH

Plasmid

#235299

Purpose

mGreenLantern expression to serve as a transfection marker, EF1a-driven

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230(R78L)

Plasmid

#225517

Purpose

Lentiviral TMEM230(R78L) expression vector

Depositor

Wade Harper

Publication

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Mutation

R78L

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230(X121PG)

Plasmid

#225518

Purpose

Lentiviral TMEM230(X121PG) expression vector

Depositor

Wade Harper

Publication

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Mutation

X121PG

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230(X121W)

Plasmid

#225519

Purpose

Lentiviral TMEM230(X121W) expression vector

Depositor

Wade Harper

Publication

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Mutation

X121W

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-TMEM230(Y29C)

Plasmid

#225520

Purpose

Lentiviral TMEM230(Y29C) expression vector

Depositor

Wade Harper

Publication

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM230 (TMEM230 Human)

Use

Lentiviral

Tags

3xHA

Mutation

Y29C

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2-bGH

Plasmid

#235269

Purpose

ComMAND base gene regulating mRuby2, EF1a

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pRPL28 CaURA3 (pNTI592)

Plasmid

#115431

Purpose

URA3-marked RPS28

Depositor

Nicholas Ingolia

Publication

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
URA3

Expression

Yeast

Mutation

KpnI site in intron

Promoter

Ashbya gossypii and endogenous

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-103 Y96F

Plasmid

#47094

Depositor

Marina Ramirez-Alvarado

Publication

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y96F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y96F

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-103 Y32F

Plasmid

#47091

Depositor

Marina Ramirez-Alvarado

Publication

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y32F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y32F

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 FL Q42K C214S

Plasmid

#47089

Depositor

Marina Ramirez-Alvarado

Publication

Levinson et al Sci Rep. 2013;3:1278. doi: 10.1038/srep0

Insert

AL-09 FL Q42K C214S (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Q42K C214S

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 FL I34N C214S

Plasmid

#47088

Depositor

Marina Ramirez-Alvarado

Publication

Levinson et al Sci Rep. 2013;3:1278. doi: 10.1038/srep0

Insert

AL-09 FL I34N C214S (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

I34N C214S

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
γA3/N-GFP

Plasmid

#33113

Depositor

Greg Phillips

Publication

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Cytoplasmic domain has been replaced with the N-c…

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pT.cruzi- 60sP0-Kn107_6xHis

Plasmid

#256087

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

60S acidic ribosomal protein P0, putative

Tags

6xHis-T7Tag-XpressTag

Expression

Bacterial

Promoter

T7

Available since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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pT.cruzi-40sS21-hypo_6xHis

Plasmid

#256078

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

40S ribosomal protein S21,hypothetical protein

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pT.cruzi-hypo-CBP_6xHis

Plasmid

#256082

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Hypothetical protein,calcium-binding protein

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pT.cruzi-Tc2-TolT_6xHis

Plasmid

#256084

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Surface protein TolT, putative

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO-Rabep1 shRNA 369958-CMV-delta5_2 Rabep1

Plasmid

#254778

Purpose

Rabep1 shRNA 369958 with expression of Rabep1 trucation lacking rab5 binding domain in pLKO backbone

Depositor

Qing Deng

Publication

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

shRNA 369958 and truncated Rabep1 (RABEP1 Human)

Use

Lentiviral

Mutation

5/2 deletion

Promoter

CMV

Available since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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