We narrowed to 31,248 results for: LIS

1. TP53-KO_gRNA_2

   Plasmid

   #195131

   Purpose

   TP53-targeting gRNA in the LRG2.1 backbone

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   TP53 KO gRNA 2 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. HH06-LP Clone 6 heavy chain

   Plasmid

   #192176

   Purpose

   Clone 6 heavy chain (HH06)

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 6 heavy chain (HH06)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLY164

   Plasmid

   #184140

   Purpose

   Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-R3, PrhaB, and Ptet

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCCL-PGK-SPdCas9-BFP-EGFP

   Plasmid

   #66821

   Purpose

   dCas9 fused to BFP and EGFP

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-EGFP
   

   Use

   Tags

   dSpCas9-BFP-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-EglN2 H358A-pBabe-puro

   Plasmid

   #22705

   Depositor

   William Kaelin

   Article

   Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

   Insert

   Egg laying Nine - 2 (EglN2) (EGLN2 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   mutated cDNA: point mutation in codon 358 changi…

   Promoter

   Available since

   Dec. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCBh_3x Flag-NLS-RhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

   Plasmid

   #197025

   Purpose

   Vector encoding a human codon-optimized RhCas12f1 driven by CBh promoter, guide RNAs compatible with RhCas12f1 driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized RhCas12f1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh, CMV, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-sgCdkn2a#1/Cre

   Plasmid

   #173629

   Purpose

   Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Cdkn2a (Cdkn2a Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-sgCdkn2a#2/Cre

   Plasmid

   #173630

   Purpose

   Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Cdkn2a (Cdkn2a Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-sgNcor1#2/Cre

   Plasmid

   #173606

   Purpose

   Expresses a Ncor1-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Ncor1 (Ncor1 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-KIF5A delta Tail

    Plasmid

    #166955

    Purpose

    Expresses FLAG-tagged KIF5A protein (1-820aa) in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    KIF5A (1-820aa)

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX028

    Plasmid

    #167151

    Purpose

    MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-nnHispS-ocsT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCardinal-Fibrillarin-7

    Plasmid

    #56160

    Purpose

    Localization: Nuclear - Nucleolus, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pMT-HGP-v3-Nb127D01-hIgG

    Plasmid

    #171564

    Purpose

    Expresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cells

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
    

    Use

    Tags

    BiP signal peptide and hIgG1-hinge-N297A-TEV site…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97310

    Purpose

    NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb NHEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-DEUT RUVBL1 Delete

    Plasmid

    #159144

    Purpose

    Expression and protien purification of mutated RUVBL1

    Depositor

    Eric Erquan Zhang

    Article

    Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

    Insert

    RUVBL1 (RUVBL1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acid 1-463, Glu134-Glu237 mutated to GPPG

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIG-707pUC19-tNGFR-P2A-TTC37

    Plasmid

    #186122

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    TTC37 (SKIC3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX018

    Plasmid

    #167144

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    fungal luciferase, nnLuz
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-sgKeap1#2/Cre

    Plasmid

    #173640

    Purpose

    Expresses a Keap1-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Keap1 (Keap1 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-sgSmad4#1/Cre

    Plasmid

    #173617

    Purpose

    Expresses a Smad4-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Smad4 (Smad4 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-sgDnmt3a#1/Cre

    Plasmid

    #173587

    Purpose

    Expresses a Dnmt3a-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Dnmt3a (Dnmt3a Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge