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(P6)TT

Plasmid

#251394

Purpose

rrnB T1 terminator derived from pTrc99A

Depositor

Kate Adamala, Pauli Kallio

Publication

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

rrnB T1 terminator derived from pTrc99A

Expression

Bacterial

Available since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSHARK6

Plasmid

#248169

Purpose

pBBR1 plasmid with constituitive lambda-CI (J23101-Bujard-Lambda-CI) and PirWT (PTet-RJ64-Pir4150) expression

Depositor

Thomas Gorochowski

Publication

Hina-Nilesh Joshi et al ACS Synth Biol. 2026 Jan 13. doi: 10.102

Insert

LambdaCI-Pir4150

Expression

Bacterial

Mutation

Pir: T108I and P113S

Available since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pESUMO-S19

Plasmid

#250656

Purpose

Plasmid for expression of E.coli S19 ribosomal protein

Depositor

Olivier Duss

Publication

Gor et al Sci Adv. 2026 Mar 20;12(12):eaec4037. do

Insert

S19 (rpsS )

Expression

Bacterial

Promoter

T7

Available since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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hSyn1-mCherry-CAAX

Plasmid

#223674

Purpose

AAV expression of mCherry from hSyn1 promoter

Depositor

Jun Nagai

Publication

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mCherry-CAAX

Use

AAV

Promoter

hSyn1

Available since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA

Plasmid

#223818

Purpose

Bacterial expression of SynPspA with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Publication

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Available since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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p366_DG

Plasmid

#234687

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and Avi tag

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

Avi tag and His10-bdNEDD8

Expression

Bacterial

Mutation

No

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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p371_DG

Plasmid

#234694

Purpose

Golden gate domesticated pET28b with Intein-Chitin binding domain

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

Intein-Chitin binding domain

Expression

Bacterial

Mutation

No

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBEST-PT7-MGapt-UTR1-deGFP

Plasmid

#227649

Purpose

pT7-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.

Depositor

Richard Murray

Insert

MG aptamer (MGapt) and deGFP

Use

Synthetic Biology

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET_SynPspA

Plasmid

#223812

Purpose

Bacterial expression of SynPspA with N-terminal 10xHis-tag and EK cleavage site

Depositor

Carsten Sachse

Publication

Junglas et al Cell. 2021 Jul 8;184(14):3674-3688.e18.

Insert

SynPspA

Tags

10xHis and NusA

Expression

Bacterial

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426

Plasmid

#226734

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Publication

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

RHOT1 (RHOT1 Human)

Use

Lentiviral

Tags

GFP11

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-GFP

Plasmid

#223888

Purpose

4G-cloning donor for C-terminal 3C-GFP tag

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3C-GFP

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-His8

Plasmid

#223887

Purpose

4G-cloning donor for C-terminal 3C-His8 tag

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3C-His8

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLP4

Plasmid

#209959

Purpose

Entry vector to store Level 0* parts

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pLP5

Plasmid

#209960

Purpose

Entry vector to store Level 0* resistance cassette parts

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pLP6

Plasmid

#209961

Purpose

Entry vector to store Level 0* replication origin parts

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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AA033

Plasmid

#216013

Purpose

Fragmid fragment: (Cas protein) deactivated Cas

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

dCas9-NG_v1.1 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA035

Plasmid

#216015

Purpose

Fragmid fragment: (Cas protein) deactivated Cas

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

dCas9-SpRY_v1.1 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA185

Plasmid

#216048

Purpose

Fragmid fragment: (C' terminus) for prime editing; using PEmax; for PE4 & 5

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

SV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1; P2A_v1.2; MLH1dn_v1.1

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA034

Plasmid

#216014

Purpose

Fragmid fragment: (Cas protein) deactivated Cas

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

dCas9-SpG_v1.1 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA197

Plasmid

#216049

Purpose

Fragmid fragment: (C' terminus) ALFA; ALFA; ALFA; ALFA; ALFA; NpNLS; 3xHA

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

ALFA_v1.3; ALFA_v1.2; ALFA_v1.1; ALFA_v1.4; ALFA_v1.5; NpNLS_v1.1; 3xHA_v1.1

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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