We narrowed to 8,725 results for: PAN

1. pAcBac1-EcTrp-TGA

   Plasmid

   #228780

   Purpose

   aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTrpRS
   EcWtR
   

   Use

   Baculoviral

   Expression

   Mammalian

   Mutation

   TGA suppressor

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSIIpuro-mNG-eDHFR(69K6)-RasGEF

   Plasmid

   #172104

   Purpose

   Mammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF-Bos TRAM Flag

   Plasmid

   #41551

   Depositor

   Kate Fitzgerald, Doug Golenbock

   Article

   Fitzgerald et al J Exp Med. 2003 Oct 6;198(7):1043-55. Ep

   Insert

   TRAM (TICAM2 Human)
   

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pTetON-DeAct-SpvB

   Plasmid

   #89455

   Purpose

   Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-SpvB (spvB Salmonella enterica)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning SpvB amino acids 375-591 (mon…

   Promoter

   TRE3G

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNK1508

   Plasmid

   #219750

   Purpose

   pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - npgA - tAOX
   

   Expression

   Yeast

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDF-Az

   Plasmid

   #197101

   Purpose

   To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

   Depositor

   Kensaku Sakamoto

   Article

   Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

   Insert

   TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
   

   Expression

   Bacterial

   Mutation

   Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV2-CAGIG-H2B-V5-mScarlet

   Plasmid

   #191093

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   H2B and mScarlet
   

   Use

   AAV

   Tags

   H2B-V5-mScarlet

   Expression

   Mammalian

   Mutation

   The fusion protein was optimized to the Human cod…

   Promoter

   CMV and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-hPhyB621-mCherry-HRasCT

   Plasmid

   #100282

   Purpose

   Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (1-621 aa) (PHYB Mustard Weed)
   

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-621 aa)

   Promoter

   CAG promoter

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMEXneo-TrkC

   Plasmid

   #190202

   Purpose

   To express the Human TrkC receptor under the Moloney mouse sarcoma virus LTR.

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

   Insert

   TrkC (NTRK3 Human)
   

   Use

   Eucaryotic

   Expression

   Mammalian

   Promoter

   Moloney mouse sarcoma virus LTR

   Available Since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2KXIG (Tol2)-tp63:ikk1C179A:GFP

    Plasmid

    #105647

    Purpose

    Expression of Ikk1(alpha) with mutated cysteine residues at position 179 in basal keratinocytes

    Depositor

    Sandra Rieger

    Article

    Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

    Insert

    tp63:ikk1C179A:GFP (chuk Zebrafish)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    p63 promoter + ikk1C179A + GFP

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. CSK_HUMAN_D0

    Plasmid

    #79683

    Purpose

    This plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSK (CSK Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pIDTSmart-CMV-PLRS1

    Plasmid

    #217365

    Purpose

    Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"

    Depositor

    Abhishek Chatterjee

    Article

    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

    Insert

    leucyl-tRNA synthetase polyspecific mutant
    

    Expression

    Mammalian

    Mutation

    M40I, T252A, Y499I, Y527A, H529G

    Promoter

    CMV

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcBac1-EcTyr-TAG

    Plasmid

    #228782

    Purpose

    EcTyr aaRS/tRNA system for mammalian ncAA incorporation

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTyrRS
    EcYtR
    BstR
    

    Use

    Baculoviral

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. p50.mALDH1A1.CD63-GFP.wpre

    Plasmid

    #226770

    Purpose

    CD63-GFP in p52 (mAldh1a backbone)

    Depositor

    Ferdinando Pucci

    Article

    Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

    Insert

    CD63 (CD63 )
    

    Expression

    Mammalian

    Available Since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSF-G1TMSNKRS

    Plasmid

    #198323

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-chPKCdelta/eDHFR(69K6)-EGFP

    Plasmid

    #178857

    Purpose

    Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-EGFP (PRKCD Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670

    Plasmid

    #178858

    Purpose

    Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_FLAG-NFL(K363TAG)

    Plasmid

    #182661

    Purpose

    Expresses mouse neurofilament light chain with a TAG codon at the position K363 & an N-terminal FLAG tag (DYKDDDDK) in mammalian cells. Can be used for genetic code expansion & click labeling of NFL.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    mouse neurofilament light chain with a K363TAG mutation (Nefl Mouse)
    

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Mutation

    K363TAG

    Promoter

    CMV

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. KC1G3_HUMAN_D0

    Plasmid

    #79685

    Purpose

    This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KC1G3 (CSNK1G3 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-lyso-pHoenix

    Plasmid

    #70112

    Purpose

    Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHoenix (Cd63 Mouse, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
    

    Tags

    Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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