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Addgene

We narrowed to 4,518 results for: GAI

Showing: 2101 - 2150 of 4518 results
  1. pNP148

    Plasmid
    #106358
    Purpose
    To insert a single copy of a construct at a site on Chr II, corresponding to a gain-of-function allele of gpa-12 expressed specifically in the adult epidermis.
    Depositor
    Insert
    col-62p_gpa-12gf_3'unc-54
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 13, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Insert
    gRNA against human ACTC1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pSB700-mouse-TTN-Cerulean

    Plasmid
    #128325
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Insert
    gRNA against mouse TTN for activation
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pSB700-TTN-Puro

    Plasmid
    #128323
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Insert
    gRNA against human TTN for activation
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pRNATU6.1 HDAC3 shRNA3

    Plasmid
    #112272
    Purpose
    HDAC3 KD
    Depositor
    Insert
    shRNA against mouse HDAC3 (Hdac3 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. Lentiviral sgRNA human CD19

    Plasmid
    #155289
    Purpose
    Lentiviral expression of sgRNA against human CD19
    Depositor
    Insert
    CD19 (CD19 Human)
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pRNATU6.1 HDAC3 shRNA2

    Plasmid
    #112271
    Purpose
    HDAC3 KD
    Depositor
    Insert
    shRNA against mouse HDAC3 (Hdac3 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pRNATU6.1 HDAC3 shRNA1

    Plasmid
    #112249
    Purpose
    HDAC3 KD
    Depositor
    Insert
    shRNA against mouse HDAC3 (Hdac3 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pSB700-mouse-ACTC1-Puro

    Plasmid
    #128326
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Insert
    gRNA against mouse ACTC1 for activation (Actc1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118158
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. PCTK1

    Plasmid
    #39053
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    PCTK1 (CDK16 Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Activation loop mutant S319D
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  12. pGKO1

    Plasmid
    #63611
    Purpose
    a binary Agrobacterium vector carrying the HSVtk gene as a negative selection marker against ectopic transformants.
    Depositor
    Type
    Empty backbone
    Use
    Binary fungal vector
    Tags
    Expression
    Mutation
    Promoter
    Lac Promoter
    Available since
    July 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  13. pKG136

    Plasmid
    #63075
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-W for protein localization and affinity purification (LAP)
    Depositor
    Insert
    CENP-W (CENPW Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    E. coli codon optimized, hardened against Dharmac…
    Promoter
    Available since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
  14. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118159
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
  15. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118160
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 2101 - 2150 of 4518 results