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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,485 results for: Coli

Showing: 841 - 860 of 9485 results

  2. pJEC626

    Plasmid
    #174367
    Purpose
    Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.
    Depositor
    James Chappell
    Article
    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793
    Insert
    Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23106
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174367-plasmid-map-sequence-id-345703

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  4. pGBW-m4136462

    Plasmid
    #149533
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149533-plasmid-map-sequence-id-307560

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  6. pGBW-m4046863

    Plasmid
    #145682
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145682-plasmid-map-sequence-id-298370

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  7. pMK110

    Plasmid
    #99535
    Purpose
    contains bgl operon sequence for E. coli in vitro transcription template
    Depositor
    Robert Landick
    Article
    Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife
    Insert
    E. coli bgl antisense region containing bglG
    Use
    Tags
    None
    Expression
    Mutation
    Promoter
    lambda Pr promoter used for in vitro transcriptio…
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    99535-plasmid-map-sequence-id-190923

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  10. pGBW-m4136453

    Plasmid
    #149535
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149535-plasmid-map-sequence-id-313669

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  11. SMT205

    Plasmid
    #134817
    Purpose
    SMT201 with mutated RBS for DHFR, in vivo assays
    Depositor
    Tanja Kortemme
    Article
    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134817-plasmid-map-sequence-id-274011

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  13. pRnr-CSD

    Plasmid
    #232578
    Purpose
    Expression of the Rnr cold shock domains I and II (residues 1 to 648) as a GST-fusion.
    Depositor
    Michael Downey
    Article
    Baijal et al bioRxiv 2025.02.12.637445
    Insert
    Rnr cold shock domains I and II (residues 1 to 648)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    232578-plasmid-map-sequence-id-472257

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  14. pYihI-N-term S-A

    Plasmid
    #233096
    Purpose
    Expression of GST-YihI with N-term serines (S) mutated to alanine (A)
    Depositor
    Michael Downey
    Article
    Baijal et al bioRxiv 2025.02.12.637445
    Insert
    YihI with N-term serines (S) mutated to alanine (A)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    N-term serines (S) mutated to alanine (A)
    Promoter
    Available since
    May 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233096-plasmid-map-sequence-id-468386

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Showing: 841 - 860 of 9485 results