We narrowed to 10,142 results for: SEC

1. Ubiq-Cas9.874W

   Plasmid

   #112686

   Purpose

   Express hSpCas9 under Ubiquitin-63E promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Use

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   Ubiquitin-63E promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. nos-Cas9.874Z1

   Plasmid

   #112685

   Purpose

   Express hSpCas9 under nanos promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Use

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   nanos

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-N-I-N-CAAX

   Plasmid

   #41686

   Purpose

   Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

   Insert

   Dronpa145N-IntersectinDH-Dronpa145N-CAAX
   

   Use

   Tags

   CAAX box

   Expression

   Mammalian

   Mutation

   K145N on Dronpa

   Promoter

   CMV

   Available since

   Dec. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSyn-ConVERGD-TVA(mChr)-W3SL

   Plasmid

   #218754

   Purpose

   Provides AND intersectional (Cre+Flp) expression of TVA-mCherry

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   TVA-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-hSyn-ConVERGD-N2cG-WPRE

   Plasmid

   #218757

   Purpose

   Provides AND intersectional (Cre+Flp) expression of N2C glycoprotein

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   N2cG
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Depositor Comments

   Promoter

   hSyn

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

   Plasmid

   #140168

   Purpose

   Expresses a regulatable secretory cargo for Tetrahymena thermophila

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   MTT1

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MTK2_007

   Plasmid

   #123702

   Purpose

   Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pEF1a
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAsp-AMA-gpdA-ptr

   Plasmid

   #191016

   Purpose

   CRISPR vector for cloning sgDNAs to target genes of several Aspergillus species (especially section Flavi)

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

   Type

   Empty backbone

   Use

   Unspecified; Aspergillus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MTK4_002

   Plasmid

   #123811

   Purpose

   Encodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   SV40 pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF-pIgH-ESCargo(FTV)

    Plasmid

    #140160

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-ConVERGD-stGtACR1(FusionRed)-W3SL

    Plasmid

    #218761

    Purpose

    Provides AND intersectional (Cre+Flp) expression of stGtACR1-FusionRed

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    stGtACR1
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDJSVT86

    Plasmid

    #108965

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

    Type

    Empty backbone

    Use

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEF-pIgH-ESCargo(FTV,Crimson)

    Plasmid

    #140162

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-hSyn-ConVERGD-ConFoff-TVA(mChr)-W3SL

    Plasmid

    #218756

    Purpose

    Provides NOT intersectional (Cre NOT Flp) expression of TVA-mCherry

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    TVA-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hSyn-ConVERGD-CoffFon-TVA(mChr)-W3SL

    Plasmid

    #218755

    Purpose

    Provides NOT intersectional (Flp NOT Cre) expression of TVA-mCherry

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    TVA-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF-pIgH-ESCargo*

    Plasmid

    #140159

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-K-I-N-CAAX

    Plasmid

    #41640

    Purpose

    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    Dronpa145K-IntersectinDH-Dronpa145N-CAAX
    

    Use

    Tags

    CAAX box

    Expression

    Mammalian

    Mutation

    K145N on Dronpa

    Promoter

    CMV

    Available since

    Dec. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF-pIgH-ESCargo(FTV,Orange)

    Plasmid

    #140161

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOCC7

    Plasmid

    #118876

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOCC154

    Plasmid

    #118902

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Use

    Tags

    TwinStrep, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge