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D5 gene in PETCON vector

Plasmid

#87626

Purpose

expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

Depositor

Sarel Fleishman

Article

Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

Insert

Designed allosteric Zn2+ and fluorescein binding antibody scFv D5

Tags

3x(G4S) Linker and cMyc tag on backbone

Expression

Yeast

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ2-(HA)3

Plasmid

#198178

Purpose

Expression of HA-tagged AP-2 μ2 in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-2 μ2

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 80

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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SNX19-GFP

Plasmid

#197392

Purpose

Expresses SNX19 in mammalian cells

Depositor

Juan Bonifacino

Article

Saric et al Nat Commun. 2021 Jul 27;12(1):4552. doi:

Insert

SNX19 (SNX19 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pME-Gal80

Plasmid

#51620

Purpose

vertebrate expression of full-length Gal80

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80 (GAL80 Budding Yeast)

Use

Vertebrate expression

Available Since

March 27, 2014

Availability

Academic Institutions and Nonprofits only

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pOBCol3.6-MCS

Plasmid

#108311

Purpose

Drive inserted gene in type 1 collagen expressing cells.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Type

Empty backbone

Tags

None

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2 xRor2-EGFP

Plasmid

#67163

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

EGFP

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pME-basGal80

Plasmid

#51621

Purpose

vertebrate expression of Gal80 preceded by a basal promoter

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80 (GAL80 Budding Yeast)

Use

Vertebrate expression

Promoter

basal promoter

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only
pM2_PLLacO1_R1_mTagBFP2

Plasmid

#250898

Purpose

mTagBFP2 expressed under PL LacO1 promoter; part of BNeu AS11*; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

mTagBFP2

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PBAD_R1_EGFP

Plasmid

#250900

Purpose

EGFP expressed under araBAD promoter; part of BNeu AS13; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PBAD

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLLacO1_R1_EGFP

Plasmid

#250897

Purpose

EGFP expressed under PL LacO1 promoter; part of BNeu AS11; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLTetO1_R1_EGFP

Plasmid

#250899

Purpose

EGFP expressed under PL TetO1 promoter; part of BNeu AS12; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL TetO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PHLT_R1_E2Crimson

Plasmid

#250886

Purpose

E2-Crimson expressed under PHLT synthetic promoter; component of BNeuAS4; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PHLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PHL_R1_EGFP

Plasmid

#250888

Purpose

EGFP expressed under PHL synthetic promoter ; part of BNeu AS8; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PHL

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLT_R1_E2Crimson

Plasmid

#250882

Purpose

E2-Crimson expression under PLT synthetic promoter; part of BNeu AS1*; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PR_R1_E2Crimson

Plasmid

#250896

Purpose

E2-Crimson expressed under PR promoter; component of Bneu AS10; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PR

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLT_R1_EGFP

Plasmid

#250881

Purpose

EGFP expression under PLT synthetic promoter; component of BNeuAS1; p15A origin

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PHT_R4_EGFP

Plasmid

#250889

Purpose

EGFP expressed under PHT synthetic promoter and regulated by RBS 'R4'; part of BNeuAS9; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PHT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ3A-(HA)3

Plasmid

#198179

Purpose

Expression of HA-tagged AP-3 μ3A in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-3 μ3A

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 225

Promoter

CMV

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-AP-4 β4

Plasmid

#198327

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 β4

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Contains L480S substitution

Promoter

ADH1

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGAD424-AP-4 σ4

Plasmid

#198328

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 σ4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 σ4

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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