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BoNT/A-LC

Plasmid

#31602

Depositor

Axel Brunger

Article

Zuniga et al Structure. 2008 Oct 8. 16(10):1588-97.

Insert

BoNT/A LC

Tags

His6

Expression

Bacterial

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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makeTCR_Lentiviral_Backbone

Plasmid

#233943

Purpose

backbone for makeTCR assembly

Depositor

Michael Platten

Type

Empty backbone

Use

Lentiviral

Promoter

EF1a (long)

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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Bongwoori-R3

Plasmid

#101658

Purpose

A variant of Bongwoori with H237R mutation in its linker region. (* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

Bongwoori-R3

Expression

Mammalian

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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Bongwoori

Plasmid

#63720

Purpose

Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D

Depositor

Bradley Baker

Article

Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

Insert

Bongwoori

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
LT1_30_backbone_X2_integration

Plasmid

#194403

Purpose

Multi Backbone with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_31_backbone_HO_integration

Plasmid

#194404

Purpose

Multi Backbone with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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makeTCR_HDR_Hs.TRAC_Backbone

Plasmid

#233944

Purpose

backbone for makeTCR assembly

Depositor

Michael Platten

Type

Empty backbone

Expression

Mammalian

Promoter

knock-in to endogenous TRAC

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPOM042_P15678_MultiGeneBackbone

Plasmid

#216467

Purpose

Empty integration vector for genomic integration for multigene into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM041_P15678_SingleGeneBackbone

Plasmid

#216466

Purpose

Empty integration vector for genomic integration of 1 transcriptional unit into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pRegev_ExonSkip_Backbone

Plasmid

#204187

Purpose

Backbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1).

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

First exon and intron for beta globin splicing minigene

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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PA-Bongwoori-R3

Plasmid

#137838

Purpose

A photoactivatable GEPI variant originated from ecliptic pHluorin

Depositor

Bradley Baker

Article

Lee et al Front Cell Neurosci. 2019 Oct 29;13:482.

Insert

Photoactivatable_Bongwoori-R3

Expression

Mammalian

Mutation

L64F, T65S, V163A, T203H, A227D

Promoter

CMV

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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Watermelon backbone

Plasmid

#155258

Purpose

Can be used to generate a Watermelon library

Depositor

Joan Brugge, Aviv Regev

Article

Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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RfxCas13d-backbone

Plasmid

#165078

Purpose

Expression of RfxCas13d sgRNAs from a U6 promoter

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

Scaffold gRNA

Expression

Mammalian

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pUNIV backbone

Plasmid

#24705

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Type

Empty backbone

Use

Xenopus oocyte expression

Expression

Bacterial, Mammalian, and Y…

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pKG1334_pHarbor-PiggyBac_backbone

Plasmid

#239705

Purpose

PaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated integration

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLys-M1 backbone

Plasmid

#109386

Purpose

Empty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmR

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Type

Empty backbone

Expression

Bacterial

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCFD3-ebony

Plasmid

#83380

Purpose

Drosophila gRNA expression plasmid targets ebony

Depositor

Richard Padgett

Article

Kane et al G3 (Bethesda). 2017 Jan 5;7(1):87-93. do

Insert

ebony (e Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pET-BoNT/X

Plasmid

#168017

Purpose

Expresses BoNT/X-LC(1-439)-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

BoNT/X-LC(1-439)-6xHis

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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CaTCH_empty-backbone

Plasmid

#157746

Purpose

Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral; Catch barcode cloning

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Barcode backbone

Plasmid

#158665

Purpose

This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Type

Empty backbone

Use

Lentiviral

Tags

hcRED

Expression

Mammalian

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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