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We narrowed to 1,121 results for: gibson assembly

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Showing: 841 - 860 of 1121 results

  1. pL0_AC-epyC

    Plasmid
    #202104
    Purpose
    Level 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Publication
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    epyC promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove bsaI sites fo…
    Available since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202104-plasmid-map-sequence-id-405424

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  13. T18m

    Plasmid
    #121037
    Purpose
    MoClo golden gate assembly DE part for L3S2P21 (382x-strength synthetic terminator with BsaI cut site removed; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S2P21 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121037-plasmid-map-sequence-id-236351

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  14. C92m

    Plasmid
    #121000
    Purpose
    MoClo golden gate assembly CD part for sfCFP-pdt3 (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with a pdt3 tag for mfLon protease).
    Depositor
    Richard Murray
    Insert
    sfCFPpdt3 (Chen,Bennett)
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121000-plasmid-map-sequence-id-238058

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  15. C101m

    Plasmid
    #121005
    Purpose
    MoClo golden gate assembly CD part sfCFP-LAA (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with LAA degradation tag for ssrA).
    Depositor
    Richard Murray
    Insert
    sfCFP with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121005-plasmid-map-sequence-id-236158

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  16. P1m

    Plasmid
    #120934
    Purpose
    MoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs.
    Depositor
    Richard Murray
    Insert
    J23101
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120934-plasmid-map-sequence-id-235942

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  18. MZB186

    Plasmid
    #228489
    Purpose
    Empty 10xHis-SUMO (Smt3) fused Avi-tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    10xHis-SUMO(Smt3)-Avitag
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228489-plasmid-map-sequence-id-455379

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  19. U22m

    Plasmid
    #121025
    Purpose
    MoClo golden gate assembly BC part (high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD6
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121025-plasmid-map-sequence-id-245900

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  20. T15m

    Plasmid
    #121034
    Purpose
    MoClo golden gate assembly DE part for L3S3P11 (173x-strength synthetic terminator, modified to remove BsaI cut site; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S3P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121034-plasmid-map-sequence-id-245426

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Showing: 841 - 860 of 1121 results