Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 5,553 results for: golden gate

Clear keywords
Showing: 841 - 860 of 5553 results

  5. C64m

    Plasmid
    #120980
    Purpose
    MoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).
    Depositor
    Richard Murray
    Insert
    temperature sensitive lambda repressor (cI857)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120980-plasmid-map-sequence-id-236034

    Enlarge

  6. C39m

    Plasmid
    #120972
    Purpose
    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdB toxin
    Use
    Synthetic Biology
    Mutation
    BsaI site removed
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120972-plasmid-map-sequence-id-236024

    Enlarge

  7. P9m

    Plasmid
    #120937
    Purpose
    MoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Pr-OR1-OR2
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120937-plasmid-map-sequence-id-247706

    Enlarge

  8. pGGG-TaBSS2-GUS(int)

    Plasmid
    #242017
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic GUS (with introns) driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    GUS_with_introns
    Expression
    Plant
    Promoter
    BSS2
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242017-plasmid-map-sequence-id-504531

    Enlarge

  9. pGGG-TaBSS2-TaSEP1B6(int)

    Plasmid
    #242016
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic SEP1-B6 driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaSEP1-B6_with_introns
    Tags
    3x FLAG
    Expression
    Plant
    Promoter
    BSS2
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242016-plasmid-map-sequence-id-504530

    Enlarge

  10. pGGG-TaBSS1-GUS(int)

    Plasmid
    #242013
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic GUS (with introns) driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    GUS_with_introns
    Expression
    Plant
    Promoter
    BSS1
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242013-plasmid-map-sequence-id-504513

    Enlarge

  11. pGGG-TaBSS1-TaSEP1B6(int)

    Plasmid
    #242012
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic SEP1-B6 driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaSEP1-B6_with_introns
    Expression
    Plant
    Promoter
    BSS1
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242012-plasmid-map-sequence-id-504511

    Enlarge

  12. pGGG-TaBSS2-TaMOFB1(int)

    Plasmid
    #242015
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic MOF-B1 driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaMOF-B1_with_introns
    Tags
    3x FLAG
    Expression
    Plant
    Promoter
    BSS2
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242015-plasmid-map-sequence-id-504508

    Enlarge

  13. pGGG-TaBSS1-TaMOFB1(int)

    Plasmid
    #242011
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic MOF-B1 driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaMOF-B1_with_introns
    Expression
    Plant
    Promoter
    BSS1
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242011-plasmid-map-sequence-id-504506

    Enlarge

  14. U4m

    Plasmid
    #121017
    Purpose
    MoClo golden gate assembly BC part for UTR1 (very strong RBS). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UTR1
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121017-plasmid-map-sequence-id-236179

    Enlarge

  15. pAGM4723:Ble:Cas9

    Plasmid
    #112208
    Purpose
    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.
    Depositor
    Thomas Mock
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    SheBle
    Use
    CRISPR; Diatom, phaeodactylum
    Tags
    Phaeodactylum fcp promoter and terminator and YFP
    Mutation
    Phaeodactylum Fcp promoter
    Promoter
    Phaeodactylum Fcp
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112208-plasmid-map-sequence-id-217248

    Enlarge

  16. pSPIN-GG

    Plasmid
    #251750
    Purpose
    Single-plasmid V. cholerae CAST encoding proteins, crRNA, and donor DNA. Entry vector: GFP dropout; BbsI spacer, BsaI promoter swap, SAPI donor cloning; pSC101* backbone.
    Depositor
    Thomas Gorochowski
    Publication
    Irvine et al Methods Mol Biol. 2026;3041:33-45. doi:
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Aug. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    251750-plasmid-map-sequence-id-514588

    Enlarge

  19. p028g13-MBP

    Plasmid
    #249631
    Purpose
    Clone MBP polypeptide in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3
    Depositor
    Isabel Abreu
    Publication
    Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi
    Insert
    N-terminal tag with MBP polypeptide
    Expression
    Bacterial
    Mutation
    Silent mutation on Gly-20 to remove a BsaI recogn…
    Available since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    249631-plasmid-map-sequence-id-504970

    Enlarge

  20. pNDGG005

    Plasmid
    #231309
    Purpose
    Vector part for BEVA; BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
    Depositor
    Barney Geddes
    Publication
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
    Expression
    Bacterial
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231309-plasmid-map-sequence-id-472146

    Enlarge

Showing: 841 - 860 of 5553 results