GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris.
Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, Sauer M, Mattanovich D, Gasser B, Marx H
BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3. PubMed Article
Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, Sauer M, Mattanovich D, Gasser B, Marx H
BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98495 | BB1_12 | empty BB1 for promoter cloning |
| 98496 | BB1_23 | empty BB1 for cds cloning |
| 98497 | BB1_34 | empty BB1 for terminator cloning |
| 98498 | BB1_12_pGAP | BB1 with insert: promoter PGAP |
| 98499 | BB1_12_pGPM1 | BB1 with insert: promoter PGPM1 |
| 98500 | BB1_12_pRPP1B | BB1 with insert: promoter PRPP1B |
| 98501 | BB1_12_pPDC1 | BB1 with insert: promoter PPDC1 |
| 98502 | BB1_12_pPOR1 | BB1 with insert: promoter PPOR1 |
| 98503 | BB1_12_pMDH3 | BB1 with insert: promoter PMDH3 |
| 98504 | BB1_12_pADH2 | BB1 with insert: promoter PADH2 |
| 98505 | BB1_12_pFBA1-1 | BB1 with insert: promoter PFBA1-1 |
| 98506 | BB1_12_pSHB17 | BB1 with insert: promoter PSHB17 |
| 98507 | BB1_12_pTEF2 | BB1 with insert: promoter PTEF2 |
| 98508 | BB1_12_pRPL2A | BB1 with insert: promoter PRPL2A |
| 98509 | BB1_12_pLAT1 | BB1 with insert: promoter PLAT1 |
| 98510 | BB1_12_pPFK300 | BB1 with insert: promoter PPpPfk |
| 98511 | BB1_12_pGUT1 | BB1 with insert: promoter PGUT1 |
| 98512 | BB1_12_pTHI11 | BB1 with insert: promoter PTHI11 |
| 98513 | BB1_12_pDAS2 | BB1 with insert: promoter PDAS2 |
| 98514 | BB1_12_pAOX1 | BB1 with insert: promoter PAOX1 |
| 98515 | BB1_12_pDAS1 | BB1 with insert: promoter PDAS1 |
| 98516 | BB1_12_pFDH1 | BB1 with insert: promoter PFDH1 |
| 98518 | BB1_23_eGFP | BB1 with insert: CDS eGFP |
| 98519 | BB1_34_ScCYC1tt | BB1 with insert: terminator ScCYC1tt |
| 98520 | BB1_34_TDH3tt | BB1 with insert: TDH3tt |
| 98521 | BB1_34_RPS2tt | BB1 with insert: RPS2tt |
| 98522 | BB1_34_RPP1Btt | BB1 with insert: RPP1Btt |
| 98523 | BB1_34_RPS17Btt | BB1 with insert: RPS17Btt |
| 98524 | BB1_34_chr4_0883tt | BB1 with insert: chr4_0883tt |
| 98525 | BB1_34_IDP1tt | BB1 with insert: IDP1tt |
| 98526 | BB1_34_RPS25Att | BB1 with insert: RPS25Att |
| 98527 | BB1_34_RPS3tt | BB1 with insert: RPS3tt |
| 98528 | BB1_34_RPL2Att | BB1 with insert: RPL2Att |
| 98529 | BB3aK_14* | empty BB3 for insertion and overexpression of 1 gene in P. pastoris |
| 98530 | BB3aK_AC | empty BB3 for insertion and overexpression of 2 genes in P. pastoris |
| 98531 | BB3aK_AD | empty BB3 for insertion and overexpression of 3 genes in P. pastoris |
| 98532 | BB3aK_AE | empty BB3 for insertion and overexpression of 4 genes in P. pastoris |
| 98533 | BB3aK_AF | empty BB3 for insertion and overexpression of 5 genes in P. pastoris |
| 98534 | BB2_AB | empty BB2 for transcription unit cloning |
| 98535 | BB2_BC | empty BB2 for transcription unit cloning |
| 98536 | BB2_CD | empty BB2 for transcription unit cloning |
| 98537 | BB2_DE | empty BB2 for transcription unit cloning |
| 98538 | BB2_EF | empty BB2 for transcription unit cloning |
| 98539 | BB2_FG | empty BB2 for transcription unit cloning |
| 98540 | BB2_GH | empty BB2 for transcription unit cloning |
| 98541 | BB2_HI | empty BB2 for transcription unit cloning |
| 98542 | BB3aZ_14* | empty BB3 for insertion and overexpression of 1 gene in P. pastoris |
| 98543 | BB3eH_14* | empty BB3 for insertion and overexpression of 1 gene in P. pastoris |
| 98544 | BB3eH_AC | empty BB3 for insertion and overexpression of 2 genes in P. pastoris |
| 98545 | BB3eH_AD | empty BB3 for insertion and overexpression of 3 genes in P. pastoris |
| 98546 | BB3eH_AE | empty BB3 for insertion and overexpression of 4 genes in P. pastoris |
| 98547 | BB3eH_AF | empty BB3 for insertion and overexpression of 5 genes in P. pastoris |
| 98548 | BB3aN_14* | empty BB3 for insertion and overexpression of 1 gene in P. pastoris |
| 98549 | BB3eN_14* | empty BB3 for insertion and overexpression of 1 gene in P. pastoris |
| 98550 | BB3rN_14* | empty BB3 for insertion and overexpression of 1 gene in P. pastoris |
| 98551 | BB3rN_AC | empty BB3 for insertion and overexpression of 2 genes in P. pastoris |
| 98552 | BB3rN_AD | empty BB3 for insertion and overexpression of 3 genes in P. pastoris |
| 98553 | BB3rN_AE | empty BB3 for insertion and overexpression of 4 genes in P. pastoris |
| 98554 | BB3rN_AF | empty BB3 for insertion and overexpression of 5 genes in P. pastoris |
| 98555 | BB3rN_AG | empty BB3 for insertion and overexpression of 6 genes in P. pastoris |
| 98556 | BB3rN_AH | empty BB3 for insertion and overexpression of 7 genes in P. pastoris |
| 98557 | BB3rN_AI | empty BB3 for insertion and overexpression of 8 genes in P. pastoris |