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GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris.
Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, Sauer M, Mattanovich D, Gasser B, Marx H
BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
98495BB1_12empty BB1 for promoter cloning
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98496BB1_23empty BB1 for cds cloning
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98497BB1_34empty BB1 for terminator cloning
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98498BB1_12_pGAPBB1 with insert: promoter PGAP
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98499BB1_12_pGPM1BB1 with insert: promoter PGPM1
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98500BB1_12_pRPP1BBB1 with insert: promoter PRPP1B
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98501BB1_12_pPDC1BB1 with insert: promoter PPDC1
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98502BB1_12_pPOR1BB1 with insert: promoter PPOR1
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98503BB1_12_pMDH3BB1 with insert: promoter PMDH3
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98504BB1_12_pADH2BB1 with insert: promoter PADH2
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98505BB1_12_pFBA1-1BB1 with insert: promoter PFBA1-1
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98506BB1_12_pSHB17BB1 with insert: promoter PSHB17
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98507BB1_12_pTEF2BB1 with insert: promoter PTEF2
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98508BB1_12_pRPL2ABB1 with insert: promoter PRPL2A
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98509BB1_12_pLAT1BB1 with insert: promoter PLAT1
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98510BB1_12_pPFK300BB1 with insert: promoter PPpPfk
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98511BB1_12_pGUT1BB1 with insert: promoter PGUT1
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98512BB1_12_pTHI11BB1 with insert: promoter PTHI11
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98513BB1_12_pDAS2BB1 with insert: promoter PDAS2
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98514BB1_12_pAOX1BB1 with insert: promoter PAOX1
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98515BB1_12_pDAS1BB1 with insert: promoter PDAS1
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98516BB1_12_pFDH1BB1 with insert: promoter PFDH1
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98518BB1_23_eGFPBB1 with insert: CDS eGFP
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98519BB1_34_ScCYC1ttBB1 with insert: terminator ScCYC1tt
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98520BB1_34_TDH3ttBB1 with insert: TDH3tt
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98521BB1_34_RPS2ttBB1 with insert: RPS2tt
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98522BB1_34_RPP1BttBB1 with insert: RPP1Btt
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98523BB1_34_RPS17BttBB1 with insert: RPS17Btt
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98524BB1_34_chr4_0883ttBB1 with insert: chr4_0883tt
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98525BB1_34_IDP1ttBB1 with insert: IDP1tt
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98526BB1_34_RPS25AttBB1 with insert: RPS25Att
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98527BB1_34_RPS3ttBB1 with insert: RPS3tt
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98528BB1_34_RPL2AttBB1 with insert: RPL2Att
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98529BB3aK_14*empty BB3 for insertion and overexpression of 1 gene in P. pastoris
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98530BB3aK_ACempty BB3 for insertion and overexpression of 2 genes in P. pastoris
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98531BB3aK_ADempty BB3 for insertion and overexpression of 3 genes in P. pastoris
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98532BB3aK_AEempty BB3 for insertion and overexpression of 4 genes in P. pastoris
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98533BB3aK_AFempty BB3 for insertion and overexpression of 5 genes in P. pastoris
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98534BB2_ABempty BB2 for transcription unit cloning
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98535BB2_BCempty BB2 for transcription unit cloning
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98536BB2_CDempty BB2 for transcription unit cloning
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98537BB2_DEempty BB2 for transcription unit cloning
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98538BB2_EFempty BB2 for transcription unit cloning
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98539BB2_FGempty BB2 for transcription unit cloning
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98540BB2_GHempty BB2 for transcription unit cloning
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98541BB2_HIempty BB2 for transcription unit cloning
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98542BB3aZ_14*empty BB3 for insertion and overexpression of 1 gene in P. pastoris
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98543BB3eH_14*empty BB3 for insertion and overexpression of 1 gene in P. pastoris
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98544BB3eH_ACempty BB3 for insertion and overexpression of 2 genes in P. pastoris
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98545BB3eH_ADempty BB3 for insertion and overexpression of 3 genes in P. pastoris
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98546BB3eH_AEempty BB3 for insertion and overexpression of 4 genes in P. pastoris
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98547BB3eH_AFempty BB3 for insertion and overexpression of 5 genes in P. pastoris
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98548BB3aN_14*empty BB3 for insertion and overexpression of 1 gene in P. pastoris
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98549BB3eN_14*empty BB3 for insertion and overexpression of 1 gene in P. pastoris
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98550BB3rN_14*empty BB3 for insertion and overexpression of 1 gene in P. pastoris
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98551BB3rN_ACempty BB3 for insertion and overexpression of 2 genes in P. pastoris
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98552BB3rN_ADempty BB3 for insertion and overexpression of 3 genes in P. pastoris
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98553BB3rN_AEempty BB3 for insertion and overexpression of 4 genes in P. pastoris
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98554BB3rN_AFempty BB3 for insertion and overexpression of 5 genes in P. pastoris
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98555BB3rN_AGempty BB3 for insertion and overexpression of 6 genes in P. pastoris
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98556BB3rN_AHempty BB3 for insertion and overexpression of 7 genes in P. pastoris
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98557BB3rN_AIempty BB3 for insertion and overexpression of 8 genes in P. pastoris
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Recombinant Antibodies from Article