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pPOTv7-g418-3mNG

Plasmid

#179832

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3x mNG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBT280

Plasmid

#122610

Purpose

express evoFERNY-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoFERNY-BE4max

Expression

Mammalian

Mutation

H102P D104N

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT280

Plasmid

#122610

Purpose

express evoFERNY-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoFERNY-BE4max

Expression

Mammalian

Mutation

H102P D104N

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG202

Plasmid

#115503

Purpose

Clone of a level 1 unit which encodes IPTG-inducible sfGFP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

pL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pOGG202

Plasmid

#115503

Purpose

Clone of a level 1 unit which encodes IPTG-inducible sfGFP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

pL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAG1188 mCherry-cagedFIREtag

Plasmid

#205177

Purpose

Expresses mCherry-cagedFIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

mCherry-FIREtag-DEVDG-MoA

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1188 mCherry-cagedFIREtag

Plasmid

#205177

Purpose

Expresses mCherry-cagedFIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

mCherry-FIREtag-DEVDG-MoA

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP

Plasmid

#84251

Purpose

Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP

Plasmid

#84251

Purpose

Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBHM2675

Plasmid

#241722

Purpose

Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source

Depositor

Hiten Madhani

Article

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2675

Plasmid

#241722

Purpose

Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source

Depositor

Hiten Madhani

Article

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ras-LOCKR-S

Plasmid

#216496

Purpose

FRET-based reporter for Ras activity, untargeted

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Ras-LOCKR-S

Expression

Mammalian

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ras-LOCKR-S

Plasmid

#216496

Purpose

FRET-based reporter for Ras activity, untargeted

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Ras-LOCKR-S

Expression

Mammalian

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pPD304 VPR-ZF43

Plasmid

#138840

Purpose

Constitutive expression of VPR-ZF43 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pPD304 VPR-ZF43

Plasmid

#138840

Purpose

Constitutive expression of VPR-ZF43 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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RFP-eNOS-NLS

Plasmid

#22496

Depositor

William Sessa

Article

Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

Insert

eNOS(G2A)-NLS

Tags

RFP

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
RFP-eNOS-NLS

Plasmid

#22496

Depositor

William Sessa

Article

Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

Insert

eNOS(G2A)-NLS

Tags

RFP

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pBT100.164

Plasmid

#138522

Purpose

phage cloning

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(7.10)-inteinN

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBT100.164

Plasmid

#138522

Purpose

phage cloning

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(7.10)-inteinN

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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1B-GFP1-10

Plasmid

#193867

Purpose

E. coli expression vector for Hisx6 tagged GFP1-10.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

sfGFP1-10

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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