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TB204 △hisD proB74

Bacterial strain

#229550

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for histidine, proline overproducer

Depositor

Yolanda Schaerli

Publication

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial resistance

None

Available since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △metB proB74

Bacterial strain

#229551

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for methionine, proline overproducer

Depositor

Yolanda Schaerli

Publication

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial resistance

None

Available since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB205 △proC △hisL

Bacterial strain

#229552

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for proline, histidine overproducer

Depositor

Yolanda Schaerli

Publication

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial resistance

None

Available since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
pQCH

Plasmid

#164160

Purpose

IncQ BHR plasmid

Depositor

Calvin Henard

Publication

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YFP Gamma 3

Plasmid

#36103

Depositor

Narasimhan Gautam

Publication

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 3 (Gng3 Mouse)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
Lentiviral CaMKKbeta K193A

Plasmid

#33323

Depositor

Anthony Means

Publication

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Lentiviral

Tags

Flag

Mutation

K194A

Promoter

human PGK

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-FLEX-SomaGCaMP7

Plasmid

#182941

Purpose

Cre-dependent expression of cell body-targeted GCaMP7f, under EF1a promoter. As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Publication

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-GCaMP7

Use

AAV

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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plenti-puro-GSDMD WT

Plasmid

#249728

Purpose

Express GSDMD WT in mammalian cells with lentiviurs

Depositor

Hao Wu

Publication

Du et al Nature. 2024 Jun;630(8016):437-446. doi:

Insert

gasdermin D (GSDMD Human)

Expression

Mammalian

Available since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-SRRM2-gRNA

Plasmid

#238251

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to SRRM2 locus

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SRRM2

Use

CRISPR

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-TCOF1-gRNA

Plasmid

#237633

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to TCOF1 locus

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TCOF1 gRNA (TCOF1 Human)

Use

CRISPR

Tags

mCherry

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CBA-hGBA1-HA-hGHpA

Plasmid

#218794

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Publication

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

CMV-CBA-hGBA1-HA-hGHpA

Use

AAV

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1-3

Plasmid

#218798

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Publication

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1-3

Expression

Mammalian

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_PT1_empty

Plasmid

#203810

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Publication

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6_PT1

Use

CRISPR

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRNA(OXTR.1)-CMV-eGFP

Plasmid

#194015

Purpose

Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

Depositor

Larry Young

Publication

Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

Inserts

sgRNA(Oxtr.1)
eGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV and u6

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIP GFP-MOSPD2 W201E

Plasmid

#186471

Purpose

Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to EGFP in mammalian cells

Depositor

Catherine Tomasetto

Publication

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

W201E (no more binding to lipid droplets)

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-ER [TM(SAC1)]

Plasmid

#186572

Purpose

Expression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cells

Depositor

Catherine Tomasetto

Publication

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

SAC1 (SACM1L Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C-terminal transmembrane region of the protein: 5…

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMX-SHP1C/S-ires-mCD8

Plasmid

#183719

Purpose

Expresses the myc-tagged SHP-1 C453S mutant (SHP-1C/S) (substrate-trapping mutant) and mouse CD8.

Depositor

Takeshi Tsubata

Publication

Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

Insert

SHP-1

Use

Retroviral

Tags

myc

Mutation

C453S

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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p1.1-mCherry

Plasmid

#162772

Purpose

Fluorescent reporter for CHO expression studies

Depositor

Ivan Vorobiev

Publication

Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

Insert

red fluorescent protein

Expression

Mammalian

Mutation

consensus Kozak sequence (GCCGCCATGG) added befor…

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p1.2-GS-eGFP

Plasmid

#162771

Purpose

Fluorescent reporter for CHO expression studies

Depositor

Ivan Vorobiev

Publication

Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

Insert

enhanced green fluorescent protein

Expression

Mammalian

Mutation

consensus Kozak sequence (GCCGCCATGG) added befor…

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B D433N

Plasmid

#171823

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N

Depositor

Veerle Baekelandt

Publication

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Promoter

CMV

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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