We narrowed to 15,991 results for: LEA

1. pTH278-GST-TEV-SUP45

   Plasmid

   #29743

   Depositor

   Tobias von der Haar

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   wild type

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-hRIPK1-Flag-S320A

   Plasmid

   #112491

   Purpose

   Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S320A

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS1-rab-3P::his24::tagBFP2

   Plasmid

   #124344

   Purpose

   Nuclear Localized Pan-Neuronal tagBFP2

   Depositor

   Andrew Leifer

   Insert

   rab3, tagBFP2 (rab-3 Nematode)
   

   Expression

   Worm

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBbi-Pur-AktAR2

   Plasmid

   #125195

   Purpose

   SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter

   Depositor

   Eric Davis

   Insert

   AktAR2
   

   Use

   Transposon

   Tags

   6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET MBP-FlAsH LIC cloning vector (MBP-FlAsH)

   Plasmid

   #30185

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), FlAsH, MBP, and TEV cleav…

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]

   Plasmid

   #86454

   Purpose

   Nuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)

   Depositor

   Gregor Bucher

   Insert

   3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
   

   Expression

   Insect

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PP-HSP90 (3PEH)

   Plasmid

   #28267

   Depositor

   Cheryl Arrowsmith

   Insert

   PP-HSP90 (PFL1070c Plasmodium Falciparum)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   contains N-terminal domain.

   Available Since

   April 14, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

8. 4YY8

   Plasmid

   #69961

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PF13_0238:MAC052-B07:C233961
   

   Use

   Baculovirus expression

   Tags

   His and TEV cleavage site

   Promoter

   polyhedrin

   Available Since

   Oct. 7, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. C67m

   Plasmid

   #120981

   Purpose

   MoClo golden gate assembly CD part for Bxb1-mScarleti-LAA (Bxb1 integrase tagged with an mScarlet-I reporter and an LAA degradation tag). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   Bxb1 integrase - mScarlet-i - LAA tag
   

   Use

   Synthetic Biology

   Tags

   ssrA degradation tag

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-hRIPK1-Flag-K377R

    Plasmid

    #112489

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K377R

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG472 H2B-iFAST

    Plasmid

    #130818

    Purpose

    Expresses H2B-iFAST in mammalian cells (nuclear labeling)

    Depositor

    Arnaud Gautier

    Insert

    H2B-iFAST
    

    Tags

    myc-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid

    #125206

    Purpose

    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter

    Depositor

    Eric Davis

    Insert

    LPAR_AKAR-WT
    

    Use

    Transposon

    Tags

    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 5C8G

    Plasmid

    #69968

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Tb427tmp.01.1830:MAC054-B05:C234722
    

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. Clim-DC1-mCherry-H2B-Venus

    Plasmid

    #129559

    Purpose

    nuclear marker

    Depositor

    Inaki Ruiz-Trillo

    Insert

    mcherry and Histone 2B fused to venus
    

    Expression

    Bacterial

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS1-rab-3P::his-24::mCherry

    Plasmid

    #124348

    Purpose

    Nuclear Localized Pan-Neuronal mCherry

    Depositor

    Andrew Leifer

    Insert

    rab-3, mCherry (rab-3 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET His6 GSSGSS NusA TEV LIC cloning vector (2NTL)

    Plasmid

    #55214

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, GSSGSS linker, NusA, and TEV cleavage site…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. PLXNA2

    Plasmid

    #28279

    Depositor

    Cheryl Arrowsmith

    Insert

    PLXNA2 (PLXNA2 Human)
    

    Tags

    His with TEV cleavage

    Expression

    Bacterial

    Mutation

    residues 1490-1600.

    Available Since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pcDNA3.1-hRIPK1-Flag-K599R

    Plasmid

    #112490

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K599R

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-hRIPK1-Flag-K115R

    Plasmid

    #112488

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K115R

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Top7 K39E K40E V48V K55E

    Plasmid

    #12464

    Purpose

    Plasmid contains Top7 K39E K40E V46A and K55E. Please note this is DIFFERENT than what the plasmid name implies.

    Depositor

    David Baker

    Insert

    Top7 K39E K40E V46A K55E
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K39E K40E V46A K55E

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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