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pRPF144

Plasmid

#106372

Purpose

C. difficile constitutive expression system. Shuttle plasmid containing constitutive Pcwp2 -gusA

Depositor

Robert Fagan, Neil Fairweather

Publication

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Pcwp2-gusA (gusA )

Use

E. coli - c. difficile shuttle vector

Mutation

codon optimised for C. difficile

Promoter

Pcwp2

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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R5 PV

Plasmid

#51734

Purpose

Dicistronic vector with PV IRES

Depositor

Vincent Mauro

Publication

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

PV IRES

Promoter

SV40

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CFP-LacI

Plasmid

#238277

Purpose

For overexpression of CFP-LacI

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CFP-LacI

Expression

Mammalian

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_ALFA-nb

Plasmid

#238262

Purpose

For overexpression of ALFA-nb

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

ALFA-nb

Expression

Mammalian

Available since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_TagBFP_P2AT2A_GFP-nb

Plasmid

#238273

Purpose

For overexpression of TagBFP_P2AT2A_GFP-nb

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TagBFP_P2AT2A_GFP-nb

Expression

Mammalian

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET22b_msfGFP_stag_KS

Plasmid

#237630

Purpose

For expression of msfGFP_stag_KS in Ecoli

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

stag_KS

Tags

msfGFP

Expression

Bacterial

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.86b.Tau2N4R

Plasmid

#226399

Purpose

Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged WT 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Publication

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT synthetic, Human)

Tags

His.3C.86b

Expression

Bacterial

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Promoter_4

Plasmid

#210630

Purpose

Luciferase experiment for TAL1 promoter 4

Depositor

Maayan Salton

Publication

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

promoter 4 (TAL1 Human)

Expression

Mammalian

Promoter

Minimal Promoter

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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P1K-3HA-Atg8∆R-MET15

Plasmid

#207034

Purpose

Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xHA

Expression

Yeast

Mutation

∆R

Promoter

pATG8

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_exon 4

Plasmid

#210631

Purpose

Luciferase experiment for TAL1 exon 4

Depositor

Maayan Salton

Publication

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 exon 4 (TAL1 Human)

Expression

Mammalian

Promoter

Minimal Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDendra2-Hygro

Plasmid

#202407

Purpose

Dendra2 expression plasmid

Depositor

Morito Kurata

Publication

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

Hygromycin

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3e- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192239

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3e

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3e-knockin-homology arm-mAID-mClover-NeoR (EIF3E Human)

Expression

Mammalian

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192242

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3f

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)

Expression

Mammalian

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192241

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3f

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3f-knockin-homology arm-mAID-mClover-NeoR (EIF3F Human)

Expression

Mammalian

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pX4

Plasmid

#183903

Purpose

donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

Depositor

Eric Marois

Publication

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

GFP

Expression

Insect

Promoter

Aedes aegypti PUb

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX3

Plasmid

#183904

Purpose

donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

Depositor

Eric Marois

Publication

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

GFP

Expression

Insect

Promoter

Aedes aegypti Polyubiquitin

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT2EFCRET(#324)

Plasmid

#184059

Purpose

cyclofen-inducible CRE activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Publication

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

CRE-ERT2

Use

Zebrafish transient transgenesis

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Xl-EF1a

Available since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-myc-BioID-hp100

Plasmid

#174738

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Publication

Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

Insert

P100 (NFKB2 Human)

Use

Retroviral

Mutation

WT

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-myc-BioID-C

Plasmid

#174737

Purpose

Overexpresion

Depositor

Shao-Cong Sun

Publication

Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

Type

Empty backbone

Use

Retroviral

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

Plasmid

#163644

Purpose

Makes a lentivirus that expresses mNeonGreen and Cas9.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

mNeonGreen-P2A-Cas9

Use

Lentiviral

Tags

mNeonGreen-P2A

Promoter

EFS

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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