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CT362

Plasmid

#233679

Purpose

Red light-inducible Cre-C; Cre-230C-PhyA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-230C-PhyA

Expression

Mammalian

Mutation

None

Promoter

CAG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT611

Plasmid

#233669

Purpose

Red light-inducible Cre-C; Cre-230C-FHY1

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-230C-FHY1

Use

Cre/Lox

Expression

Mammalian

Mutation

None

Promoter

CAGG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT367

Plasmid

#233673

Purpose

Red light-inducible Cre-N; Cre-N229-FHY1

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-N229-FHY1

Expression

Mammalian

Mutation

None

Promoter

CAG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT336

Plasmid

#233662

Purpose

AND Gate Reporter to identify Cre and Flp recombination; FRT-STOP-FRT-loxP-STOP-loxP-GFP

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

FRT-STOP-FRT-loxP-STOP-loxP-GFP

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIIS794

Plasmid

#238207

Purpose

Expresses native TaTasR TIGR locus, TasR has premature stop codon. CmR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Expression

Bacterial

Mutation

A insertion at K85 codon

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-3xAla

Plasmid

#233921

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xAla (mutated TRAF6 binding site)

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-3xAla

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-DHF

Plasmid

#233922

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-DHF (Designed Helical Filament)

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-DHF

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-1x

Plasmid

#233923

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1x which contains only 1 TRAF6 binding motif

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-1x

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-3x

Plasmid

#233925

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 3 TRAF6 binding motifs

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-3x

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-5x

Plasmid

#233927

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 5 TRAF6 binding motifs

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-5x

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS_bDD

Plasmid

#233931

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-dDD contains the bacterial death domain from Nostoc.

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-bDD

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-11xTA

Plasmid

#233932

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-11xTAcontains 11 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-11xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-15xTA

Plasmid

#233933

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-15xTAcontains 15 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-15xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-17xTA

Plasmid

#233934

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-17xTAcontains 17 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-17xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-20xTA

Plasmid

#233935

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-10xTAcontains 20 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-20xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS-TIR

Plasmid

#233919

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS): CHARMS-TIR

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS-TIR

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3220

Plasmid

#234099

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

sfGFP

Tags

FLAG

Expression

Bacterial

Mutation

S30R/Y39N/N105T/Y145F/I171V/A206V

Promoter

pJ23100-BsaI

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3237

Plasmid

#234091

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

sfGFP

Tags

FLAG

Expression

Bacterial

Mutation

S30R/Y39N/N105T/Y145F/I171V/A206V

Promoter

pJ23100-BsaI

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N73_eMagBF_V5

Plasmid

#235642

Purpose

Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5. No nuclear export sequence to allow nuclear localization.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5

Expression

Mammalian

Mutation

residues 1-73

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_Fbl_N73_eMagBF_V5

Plasmid

#235643

Purpose

Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, Fbl for targeting to nucleolus.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

Fbl-N73- and eMagBF-V5

Expression

Mammalian

Mutation

residues 1-73

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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