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  4. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...sensitivity for unitary synaptic events. Neuron, 112(22), 3680–3696.e8. doi: https://doi.org/10.1016/j.neuron...

  10. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...adeno-associated virus infection. Nature, 530(7588), 108–112. https://doi.org/10.1038/nature16465 Rubin, J. D....Translational Medicine, 5(189), 189ra76. https://doi.org/10.1126/scitranslmed.3005708 Goertsen, D., Goeden, N., ...of Virology, 75(15), 6884–6893. https://doi.org/10.1128/jvi.75.15.6884-6893.2001 Pillay, S., Meyer, N. ...of Virology, 80(18), 9093–9103. https://doi.org/10.1128/jvi.00895-06 Additional resources on the Addgene...

  12. Deep Dive: qPCR

    Type
    Blog Post
    ...RT-PCR: pitfalls and potential. BioTechniques 26:112–115.Marino JH; Cook P; and Miller KS. 2003. Accurate...2009 Apr;55(4):611-22. doi: 10.1373/clinchem.2008.112797. Epub 2009 Feb 26. PMID: 19246619.Downey N. Interpreting...281(4):951-6. doi: 10.1006/bbrc.2001.4439. PMID: 11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl...

  13. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... loci in human cells. Proc Natl Acad Sci U S A . 112(10):3002-7. PMID: 25713381 Ma H, Tu LC, Naseri A,...systems. Nat Rev Microbiol . 13(11):722-36. PMID: 26411297 Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo...

  14. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Guntas et al., Proc Natl Acad Sci USA 2015 Jan 6; 112(1):112-7. doi: 10.1073/pnas. CRISPR-Cas9 optogenetic...Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. A novel... Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380 MuLE vectors for customized ...

  15. Chemogenetics Guide

    Type
    Guide
    ... and Restore Neural Function. Cell Stem Cell. 28:112-126.e6. PMID: 32966778 (Link opens in a new window...

  16. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...effector. Science 353:aaf5573 . https://doi.org/10.1126/science.aaf5573 Broughton JP, Deng X, Yu G, Fasching...activity. Science 360:436–439 . https://doi.org/10.1126/science.aar6245 Gootenberg JS, Abudayyeh OO, Lee...CRISPR-Cas13a/C2c2. Science 356:438–442 . https://doi.org/10.1126/science.aam9321 Gootenberg JS, Abudayyeh OO, Kellner...and Csm6. Science 360:439–444 . https://doi.org/10.1126/science.aaq0179 Myhrvold C, Freije CA, Gootenberg...CRISPR-Cas13. Science 360:444–448 . https://doi.org/10.1126/science.aas8836 Niewoehner O, Jinek M (2016) Structural...

  17. Typing CRISPR Systems

    Type
    Blog Post
    ...clustering. Science, 382(6673). https://doi.org/10.1126/science.adi1910 Chylinski, K., Makarova, K. S.,.... Science, 339(6121), 819–823. https://doi.org/10.1126/science.1231143 Cox, D. B. T., Gootenberg, J. S...Science, 358(6366), 1019–1027. https://doi.org/10.1126/science.aaq0180 Harrington, L. B., Burstein, D..... Science, 362(6416), 839–842. https://doi.org/10.1126/science.aav4294 Hu, C., Myers, M. T., Zhou, X.,...systems. Science, 363(6422), 88–91. https://doi.org/10.1126/science.aav7271 Yoshimi, K., & Mashimo, T. (2022b...

  18. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg): The EF1a promoter directs strong...sensor AAV inventory here. pAAV.hSynap.iGABASnFR (112159-AAV1) Optogenetics Optogenetics continues to be...) pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5) Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE...

  19. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...Microbiol Spectr. 2015 Apr;3(2):10.1128/microbiolspec.PLAS-0036-2014. doi: 10.1128/microbiolspec.PLAS-0036-2014...Chromosomes. Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128/microbiolspec.PLAS-0025-2014. PMID: 26104344. Fishel...processes. Biotechnol Bioeng. 2009 Aug 15;103(6):1129-43. doi: 10.1002/bit.22347. PMID: 19408315; PMCID...

  20. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 111262  AAV1 pAAV-hSynapsin1-axon-GCaMP6s 112005  AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3...axon-GCaMP6s-P2A-mRuby3) 112010  AAV9 pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid...
Showing: 1 - 20 of 122 results