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  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ... luminescence imaging.” Proc Natl Acad Sci U S A 112(14) (2015): 4352-6. PMID: 25831507. PMCID: PMC4394297...

  2. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...Academy of Sciences of the United States of America 112 10 (2015): 3002-7. PubMed PMID: 25713381 PubMed Central...

  3. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...loci in human cells.” Proc. Natl. Acad. Sci. USA 112(10) (2015): 3002-7. PubMed PMID: 25713381. PubMed...

  4. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...sensitivity for unitary synaptic events. Neuron, 112(22), 3680–3696.e8. doi: https://doi.org/10.1016/j.neuron...

  6. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...National Academy of Sciences, 112(18), pp. 5844–9. doi: 10.1073/pnas.1419685112. PubMed PMID: 25902487. PubMed...

  10. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...adeno-associated virus infection. Nature, 530(7588), 108–112. https://doi.org/10.1038/nature16465 Rubin, J. D....Translational Medicine, 5(189), 189ra76. https://doi.org/10.1126/scitranslmed.3005708 Goertsen, D., Goeden, N., ...of Virology, 75(15), 6884–6893. https://doi.org/10.1128/jvi.75.15.6884-6893.2001 Pillay, S., Meyer, N. ...of Virology, 80(18), 9093–9103. https://doi.org/10.1128/jvi.00895-06 Additional resources on the Addgene...

  11. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...Proceedings of the National Academy of Sciences USA 112(11) (2015): 3570-5. PubMed PMID: 25733849. PubMed...

  12. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...RT-PCR: pitfalls and potential. BioTechniques 26:112–115.Marino JH; Cook P; and Miller KS. 2003. Accurate...2009 Apr;55(4):611-22. doi: 10.1373/clinchem.2008.112797. Epub 2009 Feb 26. PMID: 19246619.Downey N. Interpreting...281(4):951-6. doi: 10.1006/bbrc.2001.4439. PMID: 11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl...

  13. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...Guntas et al., Proc Natl Acad Sci USA 2015 Jan 6; 112(1):112-7. doi: 10.1073/pnas. CRISPR-Cas9 optogenetic...Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. A novel... Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380 MuLE vectors for customized ...

  14. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...effector. Science 353:aaf5573 . https://doi.org/10.1126/science.aaf5573 Broughton JP, Deng X, Yu G, Fasching...activity. Science 360:436–439 . https://doi.org/10.1126/science.aar6245 Gootenberg JS, Abudayyeh OO, Lee...CRISPR-Cas13a/C2c2. Science 356:438–442 . https://doi.org/10.1126/science.aam9321 Gootenberg JS, Abudayyeh OO, Kellner...and Csm6. Science 360:439–444 . https://doi.org/10.1126/science.aaq0179 Myhrvold C, Freije CA, Gootenberg...CRISPR-Cas13. Science 360:444–448 . https://doi.org/10.1126/science.aas8836 Niewoehner O, Jinek M (2016) Structural...

  15. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...clustering. Science, 382(6673). https://doi.org/10.1126/science.adi1910 Chylinski, K., Makarova, K. S.,.... Science, 339(6121), 819–823. https://doi.org/10.1126/science.1231143 Cox, D. B. T., Gootenberg, J. S...Science, 358(6366), 1019–1027. https://doi.org/10.1126/science.aaq0180 Harrington, L. B., Burstein, D..... Science, 362(6416), 839–842. https://doi.org/10.1126/science.aav4294 Hu, C., Myers, M. T., Zhou, X.,...systems. Science, 363(6422), 88–91. https://doi.org/10.1126/science.aav7271 Yoshimi, K., & Mashimo, T. (2022b...

  16. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg): The EF1a promoter directs strong...sensor AAV inventory here. pAAV.hSynap.iGABASnFR (112159-AAV1) Optogenetics Optogenetics continues to be...) pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5) Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE...

  17. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...Microbiol Spectr. 2015 Apr;3(2):10.1128/microbiolspec.PLAS-0036-2014. doi: 10.1128/microbiolspec.PLAS-0036-2014...Chromosomes. Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128/microbiolspec.PLAS-0025-2014. PMID: 26104344. Fishel...processes. Biotechnol Bioeng. 2009 Aug 15;103(6):1129-43. doi: 10.1002/bit.22347. PMID: 19408315; PMCID...

  18. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 111262  AAV1 pAAV-hSynapsin1-axon-GCaMP6s 112005  AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3...axon-GCaMP6s-P2A-mRuby3) 112010  AAV9 pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid...

  19. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Microbiological Reviews, 56(3), 375–394. https://doi.org/10.1128/MR.56.3.375-394.1992. PubMed PMID: 1406488. PubMed...of Virology, 14(5), 1132–1141. https://doi.org/10.1128/JVI.14.5.1132-1141.1974. PubMed PMID: 4372391. ...Journal of Virology, 82(5), 2376. https://doi.org/10.1128/JVI.02100-07. PubMed PMID: 18094172. PubMed Central...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...Mouse IgG1 TP1107 pTP1112 (1x cysteine) TP1122 (3x cysteines) Mouse IgG2a TP1129 pTP1005 (3x cysteines...
Showing: 1 - 20 of 96 results