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We narrowed to 4,482 results for: GAI

Showing: 2151 - 2200 of 4482 results

  1. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118157
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118157-plasmid-map-sequence-id-240364

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  5. pMZ288

    Plasmid
    #59897
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+
    Depositor
    Mikel Zaratiegui
    Article
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59897-plasmid-map-sequence-id-94870

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  6. pGKO2

    Plasmid
    #63617
    Purpose
    a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants.
    Depositor
    Seogchan Kang
    Article
    Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92
    Type
    Empty backbone
    Use
    Fungal expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    63617-plasmid-map-sequence-id-119644

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  12. pMZ289

    Plasmid
    #59898
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210
    Depositor
    Mikel Zaratiegui
    Article
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59898-plasmid-map-sequence-id-94871

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  14. pMZ381

    Plasmid
    #59899
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469
    Depositor
    Mikel Zaratiegui
    Article
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59899-plasmid-map-sequence-id-94872

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  24. pRN1120

    Plasmid
    #101750
    Purpose
    Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    101750-plasmid-map-sequence-id-199900

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  25. pBS-GMR-eya(shRNA)

    Plasmid
    #157991
    Purpose
    pBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.
    Depositor
    Greg Beitel
    Article
    Nyberg et al Fly (Austin). 2020 Oct 5. doi: 10.1080/1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    157991-plasmid-map-sequence-id-313250

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  28. pAraH6HATT

    Plasmid
    #63901
    Purpose
    For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Use
    Tags
    6xHis and HA tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    63901-plasmid-map-sequence-id-136457

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  29. lentiCRISPR - AAVS1 sgRNA

    Plasmid
    #70661
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against AAVS1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. pComb3HTT

    Plasmid
    #63900
    Purpose
    Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Use
    Phage display
    Tags
    geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63900-plasmid-map-sequence-id-119142

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  37. pLKO-Tet-On-HDAC1-shRNA2

    Plasmid
    #90241
    Purpose
    Lentivirus for expression of shRNA2 against human HDAC1 (Dox-inducible)
    Depositor
    Roland Friedel
    Insert
    HDAC1 (HDAC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    90241-plasmid-map-sequence-id-178296

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  38. pmIL-6 -231

    Plasmid
    #61287
    Purpose
    drives luciferase via mouse IL-6 promoter, truncated (-231)
    Depositor
    Gail Bishop
    Article
    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.
    Insert
    Truncated IL-6 promoter
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of 1046 nucleotides from the 5' end…
    Promoter
    IL-6 promoter (truncated)
    Available since
    May 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pLKO-Tet-On-HDAC1-shRNA1

    Plasmid
    #90242
    Purpose
    Lentivirus for expression of shRNA1 against human HDAC1 (Dox-inducible)
    Depositor
    Roland Friedel
    Insert
    HDAC1 (HDAC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    90242-plasmid-map-sequence-id-178298

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  41. pGKO2-Gateway

    Plasmid
    #63618
    Purpose
    a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants. Uses Gateway cloning.
    Depositor
    Seogchan Kang
    Article
    Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92
    Type
    Empty backbone
    Use
    Fungal expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    63618-plasmid-map-sequence-id-119646

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Showing: 2151 - 2200 of 4482 results