Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 5,985 results for: actin

Clear keywords
Showing: 861 - 880 of 5985 results

  2. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid
    #104567
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Insert
    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    Use
    CRISPR
    Tags
    NLS-3xHA-VPR
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    104567-plasmid-map-sequence-id-201429

    Enlarge

  6. pTwist-SARS-CoV-2 Δ18 B.1.1.529

    Plasmid
    #179907
    Purpose
    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Publication
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    A67V; Δ69-70; T95I; G142D; Δ143-145; N211I; Δ212;…
    Promoter
    CAG
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    19 Articles
    179907-plasmid-map-sequence-id-351282

    Enlarge

  7. pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (AAV9)

    Viral prep
    #162382-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (#162382). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of the ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    CAG
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  18. PB-SAM

    Plasmid
    #102559
    Purpose
    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.
    Depositor
    Ying Liu
    Publication
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Inserts
    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9 and N55K in MS2
    Promoter
    CAG and EF1A
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102559-plasmid-map-sequence-id-193885

    Enlarge

  19. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    107943-plasmid-map-sequence-id-207614

    Enlarge

Showing: 861 - 880 of 5985 results