We narrowed to 90,020 results for: Mal

1. pdCas9-humanized

   Plasmid

   #44246

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dead Cas9 with 3X NLS
   

   Use

   CRISPR and Retroviral; Vector

   Tags

   3x NLS

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC015 - dLwCas13a-NF

   Plasmid

   #91905

   Purpose

   Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   dLwCas13a
   

   Use

   CRISPR

   Tags

   NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

   Expression

   Mammalian

   Mutation

   R474A and R1046A

   Promoter

   CAG

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSP1830

   Plasmid

   #70708

   Purpose

   Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Insert

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   CAG

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-Ubiquitin

   Plasmid

   #18712

   Purpose

   Mammalian expression of human ubiquitin with HA tag

   Depositor

   Edward Yeh

   Article

   Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

   Insert

   ubiquitin (UBB Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The HA-Ubiquitin insert is from the UBB gene. The…

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAM-AAV-mSncg-Cre

   Plasmid

   #153207

   Purpose

   Mouse gamma-synuclein (mSncg) promoter-mediates Cre expression in retinal ganglion cells

   Depositor

   Yang Hu

   Article

   Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   mSncg

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3-N90-beta-catenin

   Plasmid

   #31785

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of aa 1-90- Constitutitvely Active

   Promoter

   EF1alpha

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4_CRE-CMVmin-luc2

   Plasmid

   #194384

   Purpose

   Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   6xCRE-CMVmin-luc2
   

   Expression

   Mammalian

   Promoter

   CMV minimal

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ColorfulCell

   Plasmid

   #62449

   Purpose

   MXS_chaining vector with 6 cassettes

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Inserts

   CMV::GTS-Citrine-bGHpA
   CMV::Lyn-Ceruleanx3-bGHpA
   CMV::NLS-TagBFPx3-bGHpA
   CMV::MTS-AzamiGreen-bGHpA
   CMV::iRFP670-SKL-bGHpA
   CMV::ETS-mCherry-KDEL-bGHpA
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

   Plasmid

   #101299

   Purpose

   FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

   Depositor

   Alpha Yap

   Article

   Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

   Insert

   alpha-Catenin
   

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-698 and 698-906 of alpha-C…

   Available Since

   June 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-dCasRx-ALKBH5-PURO

    Plasmid

    #175582

    Purpose

    Expresses dCasRx-ALKBH5 in mammalian cell

    Depositor

    Qi Xie

    Article

    Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

    Insert

    dCasRx-ALKBH5
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    RfxCas13d R239A/H244A/R858A/H863A

    Promoter

    pMSCV

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS_4xMma PylT_GFP 150TAG 6xHis

    Plasmid

    #140015

    Purpose

    Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKH3-TRIM28

    Plasmid

    #45569

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pcDNA3.1+_hsTRPA1_myc_dTom

    Plasmid

    #183179

    Purpose

    myc tagged hsTRPA1 used for cell transfection

    Depositor

    Sreekanth Chalasani

    Article

    Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

    Inserts

    hsTRPA1 (TRPA1 Human)
    dTom
    

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-OsTIR1(F74G)

    Plasmid

    #140730

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-NES

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G)-P2A-mAID-EGFP-NES
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti6/V5-p53_R273H

    Plasmid

    #22934

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R273H Mutant

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a-Cas9-2XNES-2ERT2

    Plasmid

    #120551

    Purpose

    Express Cas9, 2xNES and 2 tandem ERT2 in mammalian cells

    Depositor

    Yu Wang

    Article

    Zhao et al Mol Ther Nucleic Acids. 2018 Dec 7;13:20

    Insert

    Cas9, NES and ERT2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAM-AAV-mSncg-0.27kb-EGFP

    Plasmid

    #153185

    Purpose

    Truncated mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-TRE3G-MYCN

    Plasmid

    #104542

    Purpose

    PiggyBac vector encoding doxycycline-inducible human MYCN

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
    

    Expression

    Bacterial and Mammalian

    Promoter

    TRE3G

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

    Plasmid

    #221550

    Purpose

    Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human TBK1 (TBK1 Human)
    

    Expression

    Mammalian

    Promoter

    u6

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5_EGFP-BRD4-NUT

    Plasmid

    #238254

    Purpose

    For overexpression of EGFP-BRD4-NUT

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EGFP-BRD4-NUT
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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