We narrowed to 19,044 results for: Met

Showing: 8801 - 8820 of 19044 results

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FLAG-TKGAPDH in pLJM60

Plasmid

#154217

Purpose

Lentiviral vector for stable expression of FLAG-TK-GAPDH

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

GAPDH

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

cDNA has been codon optimized for human cell expr…

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::PuroR-bGHpA

Plasmid

#62439

Purpose

MXS_chaining vector with CMV::PuroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_nucLAMA-G97 IRES EGFP

Plasmid

#136620

Purpose

Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-G97-NLS (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR1A-3XFlag-WT-MEFV

Plasmid

#167018

Purpose

Gateway entry plasmid with 3xflag-pyrin (Wild-type)

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Gateway entry vector

Tags

3xFlag

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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Dimer cleaved

Plasmid

#198199

Purpose

FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mVenus-T2A-mTurquoise2

Expression

Mammalian

Mutation

A206K both

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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ALIX-His(1-868, P801G)

Plasmid

#186808

Purpose

Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

ALIX-His (1-868, P801G) (PDCD6IP Human)

Tags

His6

Expression

Bacterial

Mutation

Proline 801 to Glycine

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pTS1017-Tier1-OTetO7-PCMVmin-SEAP

Plasmid

#169586

Purpose

Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

TetO7-PCMVmin

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xhfYFP

Plasmid

#186544

Purpose

Hyperfolder YFP, triple fusion: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP (3X)

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDGE1108

Plasmid

#202772

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP713-P2A-GFP

Plasmid

#197163

Purpose

Expresses the protein of miRFP713-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP713-P2A-GFP

Use

AAV

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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Psicheck2 PTPRN2 full length 3'UTR

Plasmid

#26994

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

PTPRN2 Full length 3' UTR (PTPRN2 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1P shSHMT2

Plasmid

#106319

Purpose

Express shRNA targeting SHMT2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

shRNA targeting SHMT2

Use

Lentiviral and RNAi

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pUCBB-pT7-eGFP

Plasmid

#32552

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

pT7

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pJCC_039 FnCas12a

Plasmid

#171666

Purpose

For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoC

Plasmid

#193809

Purpose

CMV overexpression of ChemoC in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoC

Tags

10xHis

Expression

Mammalian

Mutation

mCerulean3:T225R; HT7: E143R, E147R, L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1280 NLS-mCherry-FIREtag-NES

Plasmid

#205172

Purpose

expresses NLS-mCherry-FIREtag-NES in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

NLS-mCherry-FIREtag-NES

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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TB:4xHRE_RE:MiniP

Plasmid

#178313

Purpose

4 copies of the HRE DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

4 copies of the heat shock DNA binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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Col1a-3'probe

Plasmid

#20731

Depositor

Rudolf Jaenisch

Article

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

Col1a-3'probe (Col1a1 Mouse)

Expression

Bacterial

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-DDK

Plasmid

#137764

Purpose

Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Tags

DDK

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRU53

Plasmid

#167679

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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