We narrowed to 14,953 results for: pET

1. pVRa16_3622

   Plasmid

   #49670

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF16_3622
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-MYC-KPTN

   Plasmid

   #87043

   Purpose

   mammalian expression of KPTN

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   KPTN (KPTN Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVRa12_807

   Plasmid

   #49663

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF12_807
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT7-V5-SBP-C1-Hs4EBP3_Z

   Plasmid

   #148151

   Purpose

   Mammalian Expression of Hs4EBP3

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

   Insert

   Hs4EBP3 (EIF4EBP3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT112_GalL_nCDA1-xBE3

   Plasmid

   #141318

   Purpose

   Expressing base editor nCDA1-xBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-xBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

   Promoter

   GalL

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PAX6 sgRNA2

   Plasmid

   #68465

   Purpose

   targeting PAX6 gene

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   sgRNA targeting PAX6 (PAX6 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAM4896 (pCV0008)

   Plasmid

   #120081

   Purpose

   Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a nourseothricin resistance gene in various cyanobacteria.

   Depositor

   Susan Golden

   Article

   Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

   Insert

   Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-nat1_A7120, CV-PconII-LTRBS-GFPmut2
   

   Use

   Other

   Tags

   Expression

   Mutation

   mobA Y25F

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV.U6.gLacZ.Cas9-T2A-GFP

   Plasmid

   #170361

   Purpose

   Negative control plasmid used for Crispr/cas9 based disruption.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

   Insert

   Cas9-T2A-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAM4889 (pCV0001)

   Plasmid

   #120079

   Purpose

   Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a spectinomycin/streptomycin resistance gene in various cyanobacteria.

   Depositor

   Susan Golden

   Article

   Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

   Insert

   Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aadA, CV-PconII-LTRBS-GFPmut2
   

   Use

   Other

   Tags

   Expression

   Mutation

   mobA Y25F

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmcsg7-mCRT(deltaP)

    Plasmid

    #83507

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

    Insert

    CALR (Calr Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    delta . Deletion of residues 187-283

    Promoter

    T7

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pmcsg7-mCRT(deltaC)

    Plasmid

    #83506

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

    Insert

    CALR (Calr Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    delta C. Contains residues 1-339

    Promoter

    T7

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pX330_rActb sgRNA / hSpCas9

    Plasmid

    #172833

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV.U6gT28.13.EFS-NS.H2B-RFP

    Plasmid

    #170388

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS-NS

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDule-Acd82

    Plasmid

    #174099

    Purpose

    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

    Depositor

    Ryan Mehl

    Article

    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

    Insert

    Acridone 82 tRNA synthetase and tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N311S, C313G, V366A, W382T

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330_Tuba1b sgRNA / hSpCas9

    Plasmid

    #172835

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVRa15_436

    Plasmid

    #49667

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF15_436
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVRa28_1088

    Plasmid

    #49683

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF28_1088
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330_rSyn1 sgRNA / hSpCas9

    Plasmid

    #172840

    Purpose

    Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVRa11_987

    Plasmid

    #49661

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF11_987
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Zebrafish-gRNA-0001

    Plasmid

    #42241

    Purpose

    gRNA targeted to zebrafish gene apoea

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-apoea (apoea Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only