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pJSK663

Plasmid

#205129

Purpose

Expression of GFP-1xPX (from SnxA) in mammalian cells

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

1xPX domain of SnxA

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTEI165

Plasmid

#126074

Purpose

Vector for expression of plastid targeted improved YFP1-10 (T65G,T205S, T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

YFP1-10 (improved)

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-blast

Plasmid

#208034

Purpose

Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-mGFP-NCOA4

Plasmid

#192802

Purpose

Stable expression of mGFP-NCOA4 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

monomeric EGFP (A206K)

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-SUN1_785

Plasmid

#125852

Purpose

Expresses SUN1_785 tagged with GFP in mammalian cells

Depositor

Miki Hieda

Article

Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

Insert

SUN1_785 (SUN1 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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Mito-SNAP

Plasmid

#206480

Purpose

Encodes a mitochondrially-targeted SNAP tag for mammalian cell expression

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

Mitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)

Tags

SNAPf

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-BI

Plasmid

#126475

Purpose

High-copy number vector for the co-expression of two genes in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-PP7v3

Plasmid

#107230

Purpose

Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

24xPP7v3

Tags

3x FLAG-tag and P2A site

Expression

Bacterial

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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Rtn3S-GFP

Plasmid

#169035

Purpose

Express human Rtn3S in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rtn3S (RTN3 Human)

Tags

acGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42(wt)

Plasmid

#12599

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (WT) (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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YIplac211-NHX1-HaloTagx2

Plasmid

#177107

Purpose

Expresses Nhx1-HaloTagx2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

NHX1 (NHX1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42(Q61L)

Plasmid

#12600

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (Q61L) (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q61L

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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YIplac211-NHX1-iGFP

Plasmid

#177108

Purpose

Expresses Nhx1-iGFP in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

NHX1 (NHX1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (BB)-2A-GFP gamma-tubulin sg

Plasmid

#104437

Purpose

Knockdown the expression of gamma-tubulin in human cells

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin sgRNA

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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HA-RNF4/pRK5

Plasmid

#59743

Purpose

Expresses RNF4 in mammalian cells

Depositor

Xiaolu Yang

Article

Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

Insert

RNF4 (RNF4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3CD

Plasmid

#174190

Purpose

Expresses PIK3CD in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3CD (PIK3CD Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Mmp11

Plasmid

#209520

Purpose

Expresses luciferase under control of Mmp11 promoter in transfected cells

Depositor

Jennifer Mitchell

Article

Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

Insert

Mmp11 promoter (Mmp11 Mouse)

Use

Luciferase

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42(T17N)

Plasmid

#12601

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (T17N) (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T17N

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-TEX264-FLAG

Plasmid

#128258

Purpose

Expresses TEX264 in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

TEX264 (TEX264 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-FLAG-FAM134Bs

Plasmid

#128261

Purpose

Expresses FAM134Bs in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

FAM134Bs (RETREG1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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