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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,986 results for: nes

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Showing: 8861 - 8880 of 18986 results

  3. pCAG-MCP-dNS-SRRM1-V5-mCherry

    Plasmid
    #235095
    Purpose
    SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domain
    Depositor
    Mitchell Guttman
    Publication
    Bhat et al Nature. 2024 May;629(8014):1165-1173. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCAG-MCP-dNS-SRRM1-V5-mCherry (SRRM1 Human)
    Expression
    Mammalian
    Mutation
    The SRRM1 entry clone from DNASU was modified usi…
    Promoter
    CAG
    Available since
    May 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    235095-plasmid-map-sequence-id-472026

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  4. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187948
    Purpose
    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187948-plasmid-map-sequence-id-373701

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  5. pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)

    Plasmid
    #49065
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Publication
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49065-plasmid-map-sequence-id-72190

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  7. pITI1

    Plasmid
    #166094
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166094-plasmid-map-sequence-id-329610

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  8. pRR2

    Plasmid
    #166097
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166097-plasmid-map-sequence-id-329109

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  16. KIFC3

    Plasmid
    #25537
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KIFC3 (KIFC3 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    ATPase domain only
    Available since
    Nov. 4, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. AA061

    Plasmid
    #216018
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for prime editing
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9-NG (H840A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216018-plasmid-map-sequence-id-431502

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  18. AA029

    Plasmid
    #216009
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for base editing
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9-NG (D10A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216009-plasmid-map-sequence-id-431424

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  19. cr3Dd

    Plasmid
    #184862
    Purpose
    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system
    Depositor
    Patrick Sobetzko
    Publication
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    Cas9, bet, exo
    Tags
    Cas9 ssrA degradation tag
    Expression
    Bacterial
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    184862-plasmid-map-sequence-id-366226

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  20. EGFP-CCDC32

    Plasmid
    #110505
    Purpose
    Fluorescent CCDC32
    Depositor
    Bernardo Mainou
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CCDC32 (CCDC32 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110505-plasmid-map-sequence-id-210723

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Showing: 8861 - 8880 of 18986 results