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pGEX-S2*HiGlpG

Plasmid

#158536

Purpose

Bacterial expression of rhomboid protease mutant S2*HiGlpG

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

S2*HiGlpG

Tags

GST tag

Expression

Bacterial

Mutation

Sector 2 mutations

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-mCherry

Plasmid

#135597

Purpose

Expression of mCherry mRNA for microinjection into S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

mCherry

Use

In vitro transcription (mrna synthesis)

Promoter

Unknown

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-XSmurf1

Plasmid

#117244

Purpose

mRNA synthesis in situ hyb probes

Depositor

Gerald Thomsen

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

Smurf1

Use

Xenopus expression

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-A-010

Plasmid

#124418

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-B-010

Plasmid

#124419

Purpose

Slot B, contains sfGFP-MarAn20 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B with sfGFP-MarAn20 reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-C-010

Plasmid

#124420

Purpose

Slot C, contains RepA70-mKate2 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C with RepA70-mKate2 reporter + terminator

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pHBSTC147

Plasmid

#124355

Purpose

Plasmid expressing inactive gp41-1 intein inserted into CcdA antitoxin

Depositor

Hideo Iwai

Article

Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

Insert

gp41-1 intein (C1G)

Tags

CcdA-C (SFAD)

Expression

Bacterial

Mutation

C1G

Promoter

STC

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBHDuet40

Plasmid

#124358

Purpose

IPTG-inducible vector encoding the N-terminal gp41-1 split fragment with N-terminally His-tagged GB1

Depositor

Hideo Iwai

Article

Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

Insert

gp41-1 N-intein

Tags

His6-GB1

Expression

Bacterial

Promoter

T7

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBHBAD42

Plasmid

#124359

Purpose

Arabinose-inducible vector encoding the C-terminal gp41-1 split fragment fused to GB1

Depositor

Hideo Iwai

Article

Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

Insert

gp41-1 C-intein

Tags

GB1 added during cloning

Expression

Bacterial

Promoter

Para

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTK587

Plasmid

#114692

Purpose

SNAP-tagging at p150's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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