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pRZ335:CMVp-BD5-AS-(3'SE)-mK2-Ex2-miRS1-BSs-pA

Plasmid

#251327

Purpose

Expression of mK2-Ex2 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex2

Expression

Mammalian

Promoter

CMVp

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR19:SSX1p-mK2-Ex1-(5'D)-ID-BD3-S-(30bp 50%GC)-(linker1)-pA

Plasmid

#251277

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR21:SSX1p-mK2-Ex1-(5'D)-ID-BD4-S-(30bp 30%GC)-(linker1)-pA

Plasmid

#251279

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR73:SSX1p-mK2-Ex1-(5'D)-AFP BD-S(50bp, 30%GC)-(linker1)-pA

Plasmid

#251285

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR13:SSX1p-mK2-Ex1-(5'D)-ID-AFP BD-S (1-30bp)-(linker1)-pA

Plasmid

#251271

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR15:SSX1p-mK2-Ex1-(5'D)-ID-BD1-S(50bp, 50%GC)-(linker1)-pA

Plasmid

#251273

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR17:SSX1p-mK2-Ex1-(5'D)-ID-BD2-S(50bp 30%GC)-(linker1)-pA

Plasmid

#251275

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR3:SSX1p-mK2-Ex1-(5'D)-ID-AFP BD-S(50bp, 30%GC)-(linker1)-pA

Plasmid

#251264

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pmMBD2-N∆GR-G pc5087

Plasmid

#229763

Purpose

Mammalian expression plasmid of MBD2-N∆GR. N-terminus of MBD2 lacking the glycine/arginine rich domain: aa75-96. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

N-terminus of MBD2 lacking the glycine/arginine r…

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2∆CC-G pc5088

Plasmid

#229764

Purpose

Mammalian expression plasmid of MBD2∆CC. Protein is lacking the coiled coil domain: aa 363-396. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Protein is lacking the coiled coil domain: aa 363…

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WTSLFD

Plasmid

#232980

Purpose

Galactose iduced expression of Gcn4 WTSLFD+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WTSLFD+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

F108W, E109T, Y110S, E111L, N112F, L113D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+HA

Plasmid

#232990

Purpose

Galactose iduced expression of Gcn4 Aro3+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro2+HA

Plasmid

#232989

Purpose

Galactose iduced expression of Gcn4 Aro2+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 S/ArotoL

Plasmid

#232979

Purpose

Galactose iduced expression of Gcn4 S/ArotoL in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 S/ArotoL

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101L, S104L, F108L, Y110L, S117L, S136L, S144L

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED

Plasmid

#232957

Purpose

Galactose iduced expression of Gcn4 ILVtoED in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123D, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoAS

Plasmid

#232959

Purpose

Galactose iduced expression of Gcn4 ILVtoAS in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoAS

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol W

Plasmid

#231878

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 solvvol W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol

Plasmid

#231877

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 solvvol

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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psiCHECK-VIM270-pCM

Plasmid

#215869

Purpose

This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

Depositor

Kumiko Ui-Tei

Article

Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

Insert

target site which is completely complementary to passanger strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)

Expression

Mammalian

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-HsNot3_607-753_U

Plasmid

#147724

Purpose

Bacterial Expression of HsNot3_607-753

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_607-753 (CNOT3 Human)

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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