We narrowed to 28,502 results for: SHI

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pX330A-1x2

Plasmid

#58766

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pTNT-B18R-6His

Plasmid

#58979

Purpose

expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Tags

His

Expression

Bacterial

Promoter

T7/Sp6

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pTNT-B18R-6His

Plasmid

#58979

Purpose

expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Tags

His

Expression

Bacterial

Promoter

T7/Sp6

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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CAG-GFP/cre

Plasmid

#49054

Purpose

Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase

Depositor

Fred Gage

Article

Tashiro et al Nat Protoc. 2006;1(6):3049-55.

Insert

GFP/Cre

Use

Retroviral

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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CAG-GFP/cre

Plasmid

#49054

Purpose

Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase

Depositor

Fred Gage

Article

Tashiro et al Nat Protoc. 2006;1(6):3049-55.

Insert

GFP/Cre

Use

Retroviral

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGEN-PspCas13b-NES-mCherry

Plasmid

#233608

Purpose

Expression of PspCas13b in mammalian cells

Depositor

Shintaro Iwasaki

Article

Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

Insert

PspCas13b-NES-mCherry

Use

CRISPR

Tags

3xFlag and mCherry

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-PspCas13b-NES-mCherry

Plasmid

#233608

Purpose

Expression of PspCas13b in mammalian cells

Depositor

Shintaro Iwasaki

Article

Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

Insert

PspCas13b-NES-mCherry

Use

CRISPR

Tags

3xFlag and mCherry

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a T7pCONS TIR-2 sfGFP

Plasmid

#154464

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a T7pCONS TIR-2 sfGFP

Plasmid

#154464

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pWUR 1+2 tetO mut89

Plasmid

#80102

Purpose

Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
Cas1 (cas1 )
Cas2 (cas2 )

Expression

Bacterial

Mutation

Q24H, P202Q, G241D, E276D, L297Q

Available Since

Dec. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pWUR 1+2 tetO mut89

Plasmid

#80102

Purpose

Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
Cas1 (cas1 )
Cas2 (cas2 )

Expression

Bacterial

Mutation

Q24H, P202Q, G241D, E276D, L297Q

Available Since

Dec. 23, 2016

Availability

Academic Institutions and Nonprofits only

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T7-VEE-IRES-Puro

Plasmid

#58970

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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T7-VEE-IRES-Puro

Plasmid

#58970

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pRK-Myc-Smurf1 delHECT

Plasmid

#13677

Depositor

Ying Zhang

Article

Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

Insert

Smurf1 deltaHect (SMURF1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Smurf1 without HECT domain (deleted amino acids 3…

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only
pRK-Myc-Smurf1 delHECT

Plasmid

#13677

Depositor

Ying Zhang

Article

Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

Insert

Smurf1 deltaHect (SMURF1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Smurf1 without HECT domain (deleted amino acids 3…

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only
pET29a-YS14

Plasmid

#66890

Purpose

Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

Depositor

Jung-Hyun Min

Article

Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

Inserts

His6-Thrombin-Xenopus laevis histones H2A
Xenopus laevis histones H2B
Xenopus laevis histones H3
Xenopus laevis histones H4-Thrombin

Tags

H2A, H2B, H3, H4, His6 tag, and Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET29a-YS14

Plasmid

#66890

Purpose

Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

Depositor

Jung-Hyun Min

Article

Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

Inserts

His6-Thrombin-Xenopus laevis histones H2A
Xenopus laevis histones H2B
Xenopus laevis histones H3
Xenopus laevis histones H4-Thrombin

Tags

H2A, H2B, H3, H4, His6 tag, and Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAGD248

Plasmid

#219498

Purpose

Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K

Expression

Bacterial

Promoter

pBAD

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAGD248

Plasmid

#219498

Purpose

Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K

Expression

Bacterial

Promoter

pBAD

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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Red Glifon 3000

Plasmid

#163116

Purpose

Red fluorescent protein-based glucose sensor for mammalian cells with lower affinity

Depositor

Takashi Tsuboi

Article

Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

Insert

Red Glifon 3000

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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