We narrowed to 11,951 results for: emb

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AA242

Plasmid

#215943

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

U6_v1; sgCD47_v1 [Sp]; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA243

Plasmid

#215944

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

U6_v1; sgCD47_v5 [Sp]; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA310

Plasmid

#215950

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control lethal

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

U6_v1; sgPLK1_v2 [Sp]; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA232

Plasmid

#215940

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

U6_v1; sgCD47_v4 [Sp]; trRNA_v14 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA238

Plasmid

#215942

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

U6_v1; sgCD47_v4 [Sp]; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA285

Plasmid

#215947

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

U6_v1; sgCD81_v1; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA048

Plasmid

#216016

Purpose

Fragmid fragment: (Cas protein) deactivated Cas

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

dCas12a (D908A)_v1.1 [EnAs]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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(F) pXYL (G) P1 (SBE216)

Plasmid

#195017

Purpose

promoter of the gene xylose reductase from R. toruloides for the position P2 (F/G)

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pXYL (G)

Expression

Bacterial

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(R) pXYL (D) P1 (SBE215)

Plasmid

#195016

Purpose

promoter of the gene xylose reductase from R. toruloides for the position P1 (R/D)

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pXYL (D)

Expression

Bacterial

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP10D-HA

Plasmid

#209234

Purpose

Mammalian expression of ATP10D

Depositor

Hye-Won Shin

Publication

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10D (ATP10D Human)

Use

Retroviral

Tags

HA

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP10B-HA

Plasmid

#209233

Purpose

Mammalian expression of ATP10B

Depositor

Hye-Won Shin

Publication

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10B (ATP10B Human)

Use

Retroviral

Tags

HA

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pML33

Plasmid

#206996

Purpose

Contains OsTiR1 ORF and LoxP and Lox2272 sites.

Depositor

Eugene Makeyev

Publication

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

OsTiR1

Expression

Mammalian

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_DE-Per_sig_pep

Plasmid

#202152

Purpose

Level 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Peroxisome signal peptide

Use

Synthetic Biology

Mutation

None

Available since

Oct. 19, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-nuc_sig_pep

Plasmid

#202135

Purpose

Level 0 plasmid containing a kozak consensus sequence and a nucleus signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Nucleus signal peptide

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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BASIC_SEVA_69.11

Plasmid

#194214

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Publication

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Expression

Bacterial

Available since

July 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Landing_Pad_8xCTS-ECFP-pA

Plasmid

#200242

Purpose

Backbone for cloning 8xCTSs (8xCRISPR-target sites)

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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U6-TM2Emp-6T_iBlue-ModFlanks

Plasmid

#200236

Purpose

Backbone for cloning longer RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvH-CtNot1optim_1676-2193_U

Plasmid

#147729

Purpose

Bacterial Expression of CtNot1optim_1676-2193

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

CtNot1optim_1676-2193

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pE5772

Plasmid

#170779

Purpose

expression of hybrid proteins in E. coli

Depositor

Anthony Schryvers

Publication

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

GentR

Tags

polyhisbioMbpTEV

Expression

Bacterial

Promoter

T7

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Dendra-SpVasa

Plasmid

#185583

Purpose

for photoconversion

Depositor

Mamiko Yajima

Publication

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

Vssa

Use

In vitro transcription (mrna synthesis)

Tags

Dendra

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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