We narrowed to 10,839 results for: yeast

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pGI3EM11

Plasmid

#135483

Purpose

Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP

Depositor

Nicolas Buchler

Publication

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunH2Bpr hph-GFP ADH1t

Tags

GFP

Expression

Bacterial and Yeast

Promoter

SpunH2Bpr

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM31

Plasmid

#135491

Purpose

Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

Depositor

Nicolas Buchler

Publication

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr hph-mCerulean3

Tags

mCerulean3

Expression

Bacterial and Yeast

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPICZ:hTRAAK(G124I)

Plasmid

#130672

Purpose

P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

Depositor

Dan Minor

Publication

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

KCNK4 (KCNK4 Human)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

N104Q, N108Q, G124I

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGWKS6

Plasmid

#139413

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mMaroon1 marker.

Depositor

James Fraser

Publication

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mMaroon1

Expression

Yeast

Promoter

TEF1

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pIRES:mTREK-1(K271Q)

Plasmid

#133273

Purpose

Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag

Depositor

Dan Minor

Publication

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

HA

Expression

Mammalian and Yeast

Mutation

K271Q

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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TEF1pro_mCherry_YFP

Plasmid

#138624

Purpose

Dual reporter backbone plasmid for CCRA experiment

Depositor

Rob Mitra

Type

Empty backbone

Expression

Yeast

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBSWHhc-p53

Plasmid

#133903

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Publication

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (TP53 Mouse)

Expression

Yeast

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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S3_002_026_096

Plasmid

#134403

Purpose

pYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

None and none

Expression

Yeast

Mutation

None and none

Promoter

pGPM1 and pRPL18B

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S3_004_026_MV1

Plasmid

#134416

Purpose

pYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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68Q-ymsfGFP

Plasmid

#128560

Purpose

Httex1 (without proline-rich region) fused to ymsfGFP

Depositor

Patrick Lajoie

Publication

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

68Q-Httex1 (without proline-rich region) fused to ymsfGFP

Tags

ymsfGFP

Expression

Yeast

Promoter

GAL1

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSK234

Plasmid

#131149

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2

Depositor

Ahmad Khalil

Publication

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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G30, Cdc20-mCherry-AID tagging

Plasmid

#130270

Purpose

Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination

Depositor

Matthias Heinemann

Publication

Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

Insert

Cdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFA CRU HISMX

Plasmid

#131164

Purpose

PCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutant

Depositor

Nils Johnsson

Publication

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

CRU

Expression

Yeast

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFA NuiHA-loxP-nat-CUP1p

Plasmid

#131170

Purpose

PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin

Depositor

Nils Johnsson

Publication

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

Nui-HA

Expression

Yeast

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1671

Plasmid

#112045

Purpose

CEN/ARS plasmid for GAL-inducible ORM1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Publication

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ORM1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA053

Plasmid

#128515

Purpose

Expresses SFA8 in Pichia

Depositor

Marcos Alcocer

Publication

Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

Insert

SFA8 a 2S albumin from sunflower (LOC110892047 Helianthus annus)

Expression

Yeast

Promoter

AOX1

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA151

Plasmid

#128526

Purpose

Expresses mutant Ber e 1 in Pichia

Depositor

Marcos Alcocer

Insert

mutant Ber e 1

Expression

Yeast

Promoter

AOX1

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMST-1312

Plasmid

#118075

Purpose

pTPI1-cexA-tCYC1

Depositor

Michael Sauer

Publication

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Insert

pTPI1-cexA-tCYC1

Expression

Yeast

Promoter

pTPI1

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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wt Cus2p

Plasmid

#73046

Purpose

Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization

Depositor

Aaron Hoskins

Publication

Rodgers et al RNA. 2015 Dec 2.

Insert

CUS2p (CUS2 Budding Yeast)

Tags

6x His tag fused to TEV cleavage site

Expression

Bacterial and Yeast

Promoter

T7

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pWS1837

Plasmid

#123058

Purpose

_-factor pheromone

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

_-factor [3-4a]

Expression

Yeast

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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