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pDRf1-4CL5-DsRed (JBEI-15461)

Plasmid

#87935

Purpose

Cloning vector for co-expressing a gene with 4CL5 in S.cerevisiae

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac(His10-SNAP-Csk)

Plasmid

#177895

Purpose

Baculo expression of His10 tag fused to SNAPtag and human Csk

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Csk (CSK Human)

Tags

10xHis-TEV-TVMV-SNAP tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG

Plasmid

#193913

Purpose

Control plasmid that expresses non-targeted miniSOG in mammalian cells

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pKG209

Plasmid

#45109

Depositor

Iain Cheeseman

Article

Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

Insert

CENP-T (aa1-374) Laci-NLS (CENPT Human)

Tags

GFP, Laci-NLS, s peptide, and tev

Expression

Mammalian

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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XE49 pt Beta-catenin-myc

Plasmid

#16840

Depositor

Randall Moon

Article

Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

Insert

pt beta-catenin-myc (ctnnb1.L Frog)

Use

Xenopus expression

Tags

6x myc

Mutation

four point mutations introduced into the GSK-3 ph…

Available Since

June 25, 2009

Availability

Academic Institutions and Nonprofits only
pL0_EF-PtBle

Plasmid

#202180

Purpose

Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

ShBle expression cassette

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMXs-EGFP-ERas-IP

Plasmid

#13826

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-BD-cop1at(335-675)

Plasmid

#44973

Depositor

Thanos Halazonetis

Article

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

BD-COP1 (COP1 Mustard Weed)

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Mutation

deleted amino acids 1-334

Promoter

pCMV

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET28-At_PRORP2-His

Plasmid

#67870

Purpose

bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP2 (PRORP2 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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C-BLa 2.0

Plasmid

#92060

Purpose

The C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

C-BLa 2.0 fusion protein

Tags

Flag (DYKDHDG)

Expression

Bacterial

Promoter

araBAD

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2b2

Plasmid

#13757

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2b2 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
GammaPCDH-A3-GFP/pEGFP-N1

Plasmid

#197343

Purpose

Plasmid expressing an antigen targeting Pan-Gamma-protocadherin-Constant

Depositor

Joshua Weiner

Article

Lobas et al J Neurochem. 2012 Mar;120(6):913-27. doi

Insert

pcdh-gamma-A3

Tags

GFP (GFP observed)

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Hyper7 in pT3TS-Dest

Plasmid

#194296

Purpose

In vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRL

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

Hyper7

Use

In vitro transcription of mrna

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn PP-HaloTag-SYT7alpha

Plasmid

#179714

Purpose

Lentiviral expression of mouse Syt7 with N terminal HaloTag

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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TAL2402

Plasmid

#36805

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

ATM-TALEN_Left (Atm Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
6xHis-TEV-UBC-9

Plasmid

#87055

Purpose

Expresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Article

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

UBC-9 (ubc-9 Nematode)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLacI-LAP-BUB1

Plasmid

#115905

Purpose

Used to express BUB1-WT fused to LacI for recruitment of BUB1 to LacO spots.

Depositor

Geert Kops

Article

Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

Insert

LacI-BUB1 (BUB1 Human)

Use

Lentiviral

Tags

EGFP

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Sec61B

Plasmid

#120244

Purpose

Expresses V5-tagged Sec61B in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Sec61B (Sec61b Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBOB-CARMIL2 BH domain-tdTomato

Plasmid

#118737

Purpose

To express CARMIL2 tagged BH with tdTomato on C-terminus

Depositor

John Cooper

Article

Lanier et al J Biol Chem. 2016 Jan 15;291(3):1076-91.

Insert

CARMIL2 BH domain (CARMIL2 Human)

Use

Lentiviral

Tags

tdTomato

Promoter

CMV

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pTransgenesis p3 sv40 minimal promoter:: Katushka RFP

Plasmid

#131731

Purpose

Transposon based transgenesis

Depositor

Rene Maehr

Article

Stanney W et al Dev Biol. 2020 Mar 15;459(2):161-180. do

Insert

SV40MinPromoter

Use

Xenopus expression

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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