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FLAG-SLC30A10-D248A

Plasmid

#82346

Purpose

Expresses FLAG-tagged SLC30A10-D248A in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D248A

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLSU4mg10

Plasmid

#126079

Purpose

Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO MycLAP-STIL ∆CC

Plasmid

#80268

Purpose

mammalian expression plasmid for MycLAP-STIL coiled-coil deletion

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Tags

Myc-GFP

Expression

Mammalian

Mutation

missing conserved coiled-coil (amino acids 721-74…

Promoter

CMV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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DYNLT1

Plasmid

#132528

Purpose

Human TCTEX-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLT1 (DYNLT1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Endolyn-delta pMEP4

Plasmid

#62962

Purpose

full length rat Endolyn cloned into delta pMEP4

Depositor

Paul Luzio

Article

Ihrke et al Biochem J. 2000 Jan 15;345 Pt 2:287-96.

Insert

Endolyn (Cd164 Rat)

Expression

Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5-MIS12-LAP-BUB1

Plasmid

#115904

Purpose

Used for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.

Depositor

Geert Kops

Article

Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

Insert

MIS12-BUB1 (MIS12 Human)

Tags

EYFP

Expression

Mammalian

Mutation

BUB1 fused to MIS12

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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prNFL-L269P-EGFP

Plasmid

#132601

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E L269P mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

806T>C, 807G>C; Leu269Pro

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-P441L-EGFP

Plasmid

#132604

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P441L mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

1322C>T, 1323A>G; Pro441Leu

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B L69E

Plasmid

#175026

Purpose

Expresses WIPI2B (L69E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (L69E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Leucine 69 to Glutamate (CTA to GAA)

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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rGSKmyc-R85-pXT7

Plasmid

#15493

Depositor

Sergei Sokol

Article

Dominguez et al Proc Natl Acad Sci U S A. 1995 Aug 29. 9

Insert

GSK-3 beta R85 (Gsk3b Rat)

Use

Xenopus rna expression

Tags

myc

Mutation

R85: K85R, dominant negative

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
TAL2322

Plasmid

#36748

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FOXO1-TALEN_Left (FOXO1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pME-hygro-hPGAP3-3HA

Plasmid

#50373

Purpose

Express C-terminally triple HA tagged human PGAP3 in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)

Tags

3x HA

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107)

Plasmid

#87202

Purpose

To express Ubl4A (90-107 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 90-107 are inserted.

Promoter

CMV

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMS28: pTns(PmcCAST)_ΔTniQ,ΔTnsD

Plasmid

#168161

Purpose

Inducible expression of PmcCAST TnsAB and TnsC proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

PmcCAST Tns proteins (TnsAB and TnsC)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS26: pTns(PmcCAST)_ΔTniQ

Plasmid

#168159

Purpose

Inducible expression of PmcCAST TnsAB, TnsC and TnsD proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

PmcCAST Tns proteins (TnsAB, TnsC and TnsD)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS27: pTns(PmcCAST)_ΔTnsD

Plasmid

#168160

Purpose

Inducible expression of PmcCAST TnsAB, TnsC and TniQ proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS25: pTns(PmcCAST)

Plasmid

#168158

Purpose

Inducible expression of PmcCAST TnsAB, TnsC, TniQ and TnsD proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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TAL2268

Plasmid

#36705

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FOXO3-TALEN_Left (Foxo3 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2269

Plasmid

#36706

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FOXO3-TALEN_Right (Foxo3 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3_PKA-CαN(E12,14K)-mPAGFP

Plasmid

#168495

Purpose

Mammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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