We narrowed to 15,130 results for: pET

1. yPet_cp229-Binder

   Plasmid

   #179137

   Purpose

   YPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   SspB
   

   Tags

   yPet_cp229

   Expression

   Mammalian

   Mutation

   Y11K/A15E, residue numbering from pdb: 1ou9

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET19-MTdTc302

   Plasmid

   #207458

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   MTdTc302 (Dntt Mouse)
   

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28-HADH2

   Plasmid

   #67863

   Purpose

   bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)

   Depositor

   Walter Rossmanith

   Article

   Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

   Insert

   HADH2 (HSD17B10 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SIRT5H158Y-pET15b

   Plasmid

   #81032

   Purpose

   Bacterial expression of Human catalytic mutant SIRT5 protein

   Depositor

   David Lombard

   Article

   Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

   Insert

   Sirt5 (SIRT5 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   H158Y catalytic mutant

   Promoter

   T7

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETM33_CLTC_Clathrin propeller

   Plasmid

   #178471

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   CLTC_Clathrin propeller (CLTC Synthetic, Human)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28_6xHis-PARP1delZn3

   Plasmid

   #173936

   Purpose

   Bacterial expression of PARP1 lacking Zn3 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn3 (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 214-373; V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETx-SR6c3

   Plasmid

   #176211

   Purpose

   Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   SR6c3
   

   Tags

   6XHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET30-2-PSPH

   Plasmid

   #83909

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   phosphoserine phosphatase (PSPH Human)
   

   Tags

   6x His tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EcYbbN pET15b

   Plasmid

   #60690

   Purpose

   Recombinant protein expression of YbbN

   Depositor

   Mark Wilson

   Article

   Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

   Insert

   YbbN
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

10. UMG-SP-1_pET29b

    Plasmid

    #215433

    Purpose

    Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-1
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. UMG-SP-2_pET29b

    Plasmid

    #215434

    Purpose

    Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-2
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET22b-EC12WTC9A

    Plasmid

    #47924

    Purpose

    Expresses E-cadherin EC12 WT with C9A mutation in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Tags

    6xHis and TEV Cleavage

    Expression

    Bacterial

    Mutation

    changed Cysteine 9 to Alanine

    Promoter

    T7

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET23a-HsUBB

    Plasmid

    #69562

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (UBB Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET28a(cd226)

    Plasmid

    #177900

    Purpose

    Bacterial expression of his10-tagged human cd226 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    CD226 (aa 288-336 only) (CD226 Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-IL-2 signal_CyPet

    Plasmid

    #128029

    Purpose

    Mammalian expression vector with N terminal IL-2 signal peptide and CyPet

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    CyPet and IL-2 signal peptide

    Expression

    Mammalian

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. USPL1cat-pETM30

    Plasmid

    #85768

    Purpose

    bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, catalytic domain (aa 212-514) (USPL1 Human)
    

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    catalytic domain (aa 212-514)

    Promoter

    T7-lac

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-Beclin1 CC

    Plasmid

    #24403

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains the CC domain (aa 151-242)

    Available Since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pET28b-Ava3855

    Plasmid

    #45743

    Depositor

    Christopher T Walsh, Emily Balskus

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    Ava3855 (Ava_3855 Anabaena variabilis)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pETDuet-1 TIM8,13

    Plasmid

    #170282

    Purpose

    Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

    Insert

    yeast Tim8 and Tim13 (TIM8 Budding Yeast)
    

    Tags

    TEV-cleavable N-terminal His tag on Tim13 (on bac…

    Expression

    Bacterial

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET30 GLTP

    Plasmid

    #170733

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

    Insert

    GLTP (GLTP Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge