We narrowed to 9,836 results for: pET

Showing: 881 - 900 of 9836 results

Results per page:

Sort results by:

pET28a-mH6-Cas12c1

Plasmid

#120872

Purpose

Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

WT

Promoter

lac

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-S9

Plasmid

#132948

Purpose

Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S9 (rpsI E.coli)

Tags

N-terminal 6xHis-tag

Expression

Bacterial

Mutation

E52C

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET:ACAT:GFP

Plasmid

#60740

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET:GCAT:GFP

Plasmid

#60736

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBz15_Strep_Rubisco

Plasmid

#140839

Purpose

wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

Form IA Rubisco; CbbL, CbbS

Tags

Strep II

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28a (+)-hTBP

Plasmid

#171073

Purpose

Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

TATA-box-binding protein (TBP Human)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-GFP(0)

Plasmid

#199166

Purpose

used to express a neutral GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(0)

Tags

MGHHHHHHGGA

Mutation

E16R, I138R, D207R, N222K, L246R

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-DUET-1-mRhubarb719-HO

Plasmid

#141200

Purpose

Expresses mRhubarb719 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb719
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

Promoter

T7

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-Gate2 ccdB

Plasmid

#117116

Purpose

Destination vector

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Type

Empty backbone

Use

Backbone for golden gate assembly for gene target…

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PesaR-Clux

Plasmid

#47658

Purpose

PesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

PesaR-C

Use

Synthetic Biology

Expression

Bacterial

Mutation

Additional esa box between -35 site and the origi…

Promoter

PesaR-C

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
pET-Duet1_6xHis-TEV-eGFP-P62

Plasmid

#190929

Purpose

Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

Depositor

Sascha Martens

Article

Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

Insert

P62 (SQSTM1 Human)

Tags

EGFP

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-26b-NbVHH05-ALFA-His

Plasmid

#171569

Purpose

Bacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-ALFA-His (UBE2J1 Human)

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-His6-Sumo-CfaC-MBP

Plasmid

#126018

Purpose

Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)

Depositor

Thomas Muir

Insert

His6-Sumo-CfaC-MBP

Tags

6xHis-SUMO and HA-MBP

Expression

Bacterial

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-deSpCas9-VP64-6xHis

Plasmid

#92117

Purpose

Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive eSpCas9-NLS-3xFLAG-VP64

Use

CRISPR

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A, H840A, K848A, K1003A, R1060A

Promoter

T7

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-(pos14)GFP

Plasmid

#89252

Purpose

Expresses GFP with +14 theoretical charge in E.Coli

Depositor

David Liu

Article

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos16)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEThT-CAHS1

Plasmid

#90027

Purpose

To express His-tagged CAHS1 in bacteria

Depositor

Takekazu Kunieda

Article

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

CAHS1

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET23a-HsRPS27a

Plasmid

#69561

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

RPS27A (RPS27A Human)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-HsSENP3

Plasmid

#71467

Purpose

Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 3 (SENP3 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET23a-Mm/HsUBA52

Plasmid

#69560

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBA52 (UBA52 Human)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-Hisx6-Sirt6

Plasmid

#20277

Depositor

Leonard Guarente

Article

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Tags

Hisx6

Expression

Bacterial

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only