We narrowed to 6,650 results for: tac

1. AEB-TMR10pUC19

   Plasmid

   #63858

   Purpose

   This pUC19 vector contains a tetramethylrosamine (TMR) binding riboswitch, controlling the translation initaition rate of luciferase gene.

   Depositor

   Howard Salis

   Article

   Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

   Insert

   TMR10 TMR riboswitch
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   tac

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pGEXndoE(E662Q)

   Plasmid

   #47716

   Purpose

   Expresses GST-EndoE(E662Q) from E. faecalis in E. coli

   Depositor

   Vincent Fischetti, Mattias Collin

   Article

   Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

   Insert

   ndoE
   

   Use

   Tags

   GST-EndoE(E186Q) and Glutathione S-transferase

   Expression

   Bacterial

   Mutation

   Changed Glu-662 to Gln

   Promoter

   tac

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFM115

   Plasmid

   #40161

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-3C (par-3 Nematode)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C terminal portion of protein (aa850-1286)

   Promoter

   tac

   Available since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

4. polySUMO3

   Plasmid

   #221067

   Purpose

   Expression of polySUMO3 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   Five repeats of SUMO3 each separated by (GGS)4 (SUMO3 Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Dcp2 H237A-EGFP

   Plasmid

   #201425

   Purpose

   Bacterial protein expression of Dcp2 H237A

   Depositor

   Michael Rosen

   Article

   Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Dcp2 (DCP2 Budding Yeast)
   

   Use

   Tags

   EGFP, TEV, 6xHis and MBP, TEV

   Expression

   Bacterial

   Mutation

   H237A

   Promoter

   tac

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Edc3-EGFP

   Plasmid

   #201419

   Purpose

   Bacterial protein expression of Edc3

   Depositor

   Michael Rosen

   Article

   Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Edc3 (EDC3 Budding Yeast)
   

   Use

   Tags

   EGFP, TEV, 6xHis and MBP, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Dcp2-EGFP

   Plasmid

   #201417

   Purpose

   Bacterial protein expression of Dcp2

   Depositor

   Michael Rosen

   Article

   Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Dcp2 (DCP2 Budding Yeast)
   

   Use

   Tags

   EGFP, TEV, 6xHis and MBP, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Dcp1 H206A-EGFP

   Plasmid

   #201424

   Purpose

   Bacterial protein expression of Dcp1 H206A

   Depositor

   Michael Rosen

   Article

   Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Dcp1 (DCP1 Budding Yeast)
   

   Use

   Tags

   EGFP, TEV, 6xHis and MBP, TEV

   Expression

   Bacterial

   Mutation

   H206A

   Promoter

   tac

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Dcp1-EGFP

   Plasmid

   #201416

   Purpose

   Bacterial protein expression of Dcp1

   Depositor

   Michael Rosen

   Article

   Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Dcp1 (DCP1 Budding Yeast)
   

   Use

   Tags

   EGFP, TEV, 6xHis and MBP, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dcp1 H198A-EGFP

    Plasmid

    #201423

    Purpose

    Bacterial protein expression of Dcp1 H198A

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dcp1 (DCP1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    H198A

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Dcp1 H43A-EGFP

    Plasmid

    #201422

    Purpose

    Bacterial protein expression of Dcp1 H43A

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dcp1 (DCP1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    H43A

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Pat1-EGFP

    Plasmid

    #201420

    Purpose

    Bacterial protein expression of Pat1

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Pat1 (PAT1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Dcp1 H40A-EGFP

    Plasmid

    #201421

    Purpose

    Bacterial protein expression of Dcp1 H40A

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dcp1 (DCP1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    H40A

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Pat1-mCh

    Plasmid

    #201415

    Purpose

    Bacterial protein expression of Pat1

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Pat1 (PAT1 Budding Yeast)
    

    Use

    Tags

    MBP, TEV and mCherry, TEV, 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FKBP-EGFP-PML peptide

    Plasmid

    #175247

    Purpose

    Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    PML (PML Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Intersectin SH3 domain B

    Plasmid

    #47397

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain B
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    SH3 domain B

    Promoter

    tac

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

17. Intersectin SH3 domain C

    Plasmid

    #47398

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain C
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    SH3 domain C

    Promoter

    tac

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

18. Intersectin SH3 domain E

    Plasmid

    #47400

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain E
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    SH3 domain E

    Promoter

    tac

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pMAL-SsoPCNA3

    Plasmid

    #107069

    Purpose

    Expresses S. solfataricus PCNA3 fused to MBP in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA3
    

    Use

    Tags

    6xHis-thrombin cleavage site and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX6P1-GCN4cc-Erp2CT

    Plasmid

    #73923

    Purpose

    GST fusion- coiled coil- Erp2 cytoplasmic tail

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Erp2 CT
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge