We narrowed to 36,022 results for: CaS

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pLV-EF1A-PARP14-1535-1601-Hygro

Plasmid

#238100

Purpose

Expression of structurally predicted WWE domain of PARP14 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP14 (PARP14 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1535-1601 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-TRIP12(749-836)-EGFP

Plasmid

#196229

Purpose

EGFP fused to the C-terminus of a WWE domain within TRIP12 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged TRIP12(749- 836)

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-DTX2(8-97)-EGFP

Plasmid

#196230

Purpose

EGFP fused to the C-terminus of first WWE domain within Deltex2 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged DTX2(8-97)

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-DTX2(8-174)-EGFP

Plasmid

#196232

Purpose

EGFP fused to the C-terminus of tandem WWE domains within Deltex2 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX2(8-174)-EGFP

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP

Plasmid

#196235

Purpose

EGFP fused to the C-terminus of Af1521(K35E/Y145R) macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged Af1521(K35E/Y145R)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

K35E and Y145R

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-RNF146(100-182)-myc

Plasmid

#196236

Purpose

RNF146(100-182) WWE domain with a myc tag fused to the C terminus & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146(100-182) encoding a C-terminal myc tag

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-RNF146(92-168)-EGFP

Plasmid

#196228

Purpose

EGFP fused to the C-terminus of a WWE domain within RNF146 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146(92-168)-EGFP

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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Gab2-MSCV-IRES-GFP

Plasmid

#240126

Purpose

Expresses Gab2 (mouse) in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

Gab2 (Gab2 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-48xS100A10 array

Plasmid

#236381

Purpose

Expression of array of 48 crRNAs targeting S100A10

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

48xS100A10 crRNA array (S100A10 Human)

Use

CRISPR and Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHR TRE_TGFbeta3_IRES_mCherry-PGK_BFP

Plasmid

#223548

Purpose

Inducible TGF beta 3 and mCherry; constitutive BFP

Depositor

Jonathan Brunger

Article

Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

Inserts

TGF beta 3 (TGFB3 Human)
mCherry
TagBFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ 3xFLAG-FEM1B C186S

Plasmid

#230995

Purpose

Transient overexpression of mouse FEM1B in mammalian cells. Mutation abolishes substrate binding.

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Insert

FEM1B (Fem1b Mouse)

Tags

3xFLAG

Expression

Mammalian

Mutation

mutated Cys186 to Ser

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ 3xFLAG-FEM1B L597A

Plasmid

#230994

Purpose

Transient overexpression of catalytic null mouse FEM1B in mammalian cells. Mutation abolishes interaction with CUL2.

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Insert

FEM1B (Fem1b Mouse)

Tags

3xFLAG

Expression

Mammalian

Mutation

mutated Leu597 to Ala

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pRGEB32-CrActin-35S

Plasmid

#226708

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from CaMV 35S promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrActin

Use

CRISPR

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB32-CrActin-UBI

Plasmid

#226709

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrActin

Use

CRISPR

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCHC025 (∆CBBp)

Plasmid

#226203

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for megaplasmid copy of the CBB operonthe CBB operon (deltaCBBp).

Use

Synthetic Biology

Mutation

∆cbbR ∆cbbLpSpXpYpEpFpPpTpZpGpKpAp

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC024 (∆SH)

Plasmid

#226202

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for soluble hydrogenase operon

Use

Synthetic Biology

Mutation

∆hoxFUYHWI ∆hypA2B2F2

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC023 (∆MBH)

Plasmid

#226201

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for membrane bound hydrogenase operon

Use

Synthetic Biology

Mutation

∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC027 (∆pemK)

Plasmid

#226204

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Upstream/downstream homology arms for addiction system toxinPemK.

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Q54A

Plasmid

#217912

Purpose

A plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutation

Depositor

Cole DeForest

Article

Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

Insert

PXP Q54A

Tags

6x His (N and C term)

Expression

Bacterial

Mutation

Glutamine at position 55 and 267 mutated to Alani…

Promoter

T5

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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T40A

Plasmid

#217911

Purpose

A plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutation

Depositor

Cole DeForest

Article

Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

Insert

PXP T40A

Tags

6x His (N and C term)

Expression

Bacterial

Mutation

Threonine at position 41 and 253 mutated to Alani…

Promoter

T5

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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