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pVRc20_992

Plasmid

#49739

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS20_992

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pBEG R3-miRNA(ccdB)-L4 #BGV228

Plasmid

#48953

Purpose

To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway cloning vector

Promoter

na

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
KX002: pMVP/PB-DEST

Plasmid

#121869

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB331

Plasmid

#68659

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Emerald Luc fused with PEST sequence [ELuc(PEST)]

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pAT-9218

Plasmid

#124225

Purpose

Bacterial SpCas9 expression

Depositor

Ervin Welker

Article

Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

Insert

SpCas9

Use

CRISPR

Expression

Bacterial

Promoter

TetR/TetA

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP1223

Plasmid

#119261

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1221

Plasmid

#119260

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSb_init containing the TM287/288 nanobody Nb_TM#2

Plasmid

#132698

Purpose

expresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

nanobody Nb_TM#2

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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M13cp-dg1 hp

Plasmid

#218093

Purpose

Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pI

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Type

Empty backbone

Expression

Bacterial

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCB-CBEv2

Plasmid

#221139

Purpose

Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

lacIq-Ptac

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDGE479

Plasmid

#153270

Purpose

M4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB316

Plasmid

#68644

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged tdTomato (tdTo…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSL0426 (CRISPR with spc3)

Plasmid

#130642

Purpose

Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

CRISPR(spacer-3)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pOBCol3.6-GFPtpz

Plasmid

#110211

Purpose

Labels immature osteoblasts and connective tissue cells expressing type 1 collagen.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPtopaz

Tags

None

Expression

Mammalian

Mutation

None

Promoter

3.6 kb rat type 1 collagen + first intron

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDGE463

Plasmid

#153234

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP1363

Plasmid

#119279

Purpose

for stabilizing ICE in the presence of rapI expression

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBsVchCAST

Plasmid

#203812

Purpose

Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_human-4xUAS

Plasmid

#125150

Purpose

Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Stap-seq screening vector

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK121

Plasmid

#110581

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

PA3 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046799

Plasmid

#145577

Purpose

Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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