We narrowed to 12,131 results for: LEA

Showing: 9021 - 9040 of 12131 results

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pX2-IFl_Con-IFr

Plasmid

#192217

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X2)

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DXO

Plasmid

#177992

Purpose

For gateway cloning of DXO

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

DXO (DXO Human)

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-N-GFP-NES

Plasmid

#222606

Purpose

Expression of MAC3-tagged GFP-NES (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' nuclear exclusion sequence (NES)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-N-GFP-NLS

Plasmid

#222607

Purpose

Expression of MAC3-tagged GFP-NLS (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' nuclear localizaition sequence (NLS)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-C-GFP-NES

Plasmid

#222603

Purpose

Expression of MAC3-tagged GFP-NES (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' nuclear exclusion sequence (NES)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET28_human-pro-IL-18_E192K_D193K

Plasmid

#214322

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

E192K_D193K

Promoter

T7

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_I48N

Plasmid

#214323

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

I48N

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pColdI-GST-mGTSF2

Plasmid

#215714

Purpose

Bacterial expression of GST-tagged GTSF2

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF2 (Gtsf2 Mouse)

Tags

6x His and GST

Expression

Bacterial

Promoter

CspA

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_LES33AAA

Plasmid

#214326

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

LES33AAA

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_DDEN29ENGD

Plasmid

#214325

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

DDEN29ENGD

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_V47N_I48N

Plasmid

#214324

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

V47N_I48N

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-4_lemur_catalytic domain

Plasmid

#214328

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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hPCNA-C148S

Plasmid

#190939

Purpose

untagged human PCNA with a cysteine to serine mutation

Depositor

Brian Kelch

Article

Magrino et al J Biol Chem. 2023 May;299(5):104656. doi

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Expression

Bacterial

Mutation

changed cysteine 148 to serine

Promoter

T7

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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MNEAP1ec

Plasmid

#131020

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

MNEAP1

Use

Gateway Cloning

Tags

eGFP-FLAG-HA

Available Since

May 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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MKAKU41ec

Plasmid

#131014

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

MKAKU41

Use

Gateway Cloning

Tags

mCherry-FLAG-HA

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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NCH1ec

Plasmid

#131016

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

NCH1

Use

Gateway Cloning

Tags

eGFP-FLAG-HA

Available Since

May 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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NCH2ec

Plasmid

#131018

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

NCH2

Use

Gateway Cloning

Tags

eGFP-FLAG-HA

Available Since

May 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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lacI-Ptac-lacO-IMBB4-pUC

Plasmid

#170770

Purpose

test plasmid for 5RM assembly, expresses lacI repressor

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Insert

lacI

Use

Synthetic Biology

Promoter

lacI promoter

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.S.V5_mCherry-NLS

Plasmid

#178235

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.S.V5 and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.Ollas.V5_mCherry-NLS

Plasmid

#178233

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.Ollas.V5 and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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