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We narrowed to 63,052 results for: cloning

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Showing: 9061 - 9080 of 63052 results

  1. PMXS-GOT1

    Plasmid
    #72872
    Purpose
    The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.
    Depositor
    David Sabatini
    Publication
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    GOT1 (GOT1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    sgRNA resistant human GOT1, 7 silent mutations c1…
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  8. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Publication
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

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  9. pC-HYG-YR

    Plasmid
    #61765
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Publication
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    Hygromycin
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Promoter
    trpC promoter
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61765-plasmid-map-sequence-id-112551

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  11. Hs.NRAS Q61K

    Plasmid
    #83180
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61K
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    83180-plasmid-map-sequence-id-158143

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  12. Hs.NRAS Q61R

    Plasmid
    #83179
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61R
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    83179-plasmid-map-sequence-id-158142

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  13. R777-E347 Hs.TIAM1

    Plasmid
    #70631
    Purpose
    Gateway ORF clone of human TIAM1 [NM_003253.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    TIAM1 (TIAM1 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    70631-plasmid-map-sequence-id-349435

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  14. R777-E318 Hs.SOS1-nostop

    Plasmid
    #70602
    Purpose
    Gateway ORF clone of human SOS1 [NM_005633.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SOS1 (SOS1 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70602-plasmid-map-sequence-id-126051

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  15. R777-E229 Hs.RASA2

    Plasmid
    #70513
    Purpose
    Gateway ORF clone of human RASA2 [NM_006506.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASA2 (RASA2 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70513-plasmid-map-sequence-id-176466

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  16. R777-E084 Hs.FGFR1-nostop

    Plasmid
    #70368
    Purpose
    Gateway ORF clone of human FGFR1 [NM_001174063.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    FGFR1 (FGFR1 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70368-plasmid-map-sequence-id-125817

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  17. R777-E085 Hs.FGFR2

    Plasmid
    #70369
    Purpose
    Gateway ORF clone of human FGFR2 [NM_000141.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    FGFR2 (FGFR2 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70369-plasmid-map-sequence-id-125818

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  20. pBP-T7_RBS-His6-Thrombin

    Plasmid
    #72979
    Purpose
    T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)
    Depositor
    Paul Freemont
    Publication
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    T7+RBS+His6+thrombin
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 9061 - 9080 of 63052 results