We narrowed to 62,357 results for: cloning

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pND003

Plasmid

#200949

Purpose

Yeast surface display destination vector for Golden Gate cloning

Depositor

Timothy Whitehead

Article

Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

Type

Empty backbone

Use

Yeast surface display

Tags

AGA2, HA, and Myc

Expression

Yeast

Promoter

GAL1

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP2846

Plasmid

#160676

Purpose

Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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BBa_J72114-BBa_J72100

Plasmid

#40781

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

lacZ

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

Plasmid

#139094

Purpose

Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

KRAB-dSpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMB.Pos2

Plasmid

#234584

Purpose

Moclo Baculo toolkit level 1 backbone with ConL1/ConR2 and Tn7L

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Tags

GFP

Expression

Insect

Mutation

GentR R98K

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMB.Pos5

Plasmid

#234587

Purpose

Moclo Baculo toolkit level 1 backbone with ConL4/ConR5 and Tn7L

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Tags

GFP

Expression

Insect

Mutation

GentR R98K

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMB.Pos1

Plasmid

#234583

Purpose

Moclo Baculo toolkit level 1 backbone with ConLS/ConR1 and Tn7L

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Tags

GFP

Expression

Insect

Mutation

GentR R98K

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMB.Pos3

Plasmid

#234585

Purpose

Moclo Baculo toolkit level 1 backbone with ConL2/ConR3 and Tn7L

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Tags

GFP

Expression

Insect

Mutation

GentR R98K

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMB.Pos4

Plasmid

#234586

Purpose

Moclo Baculo toolkit level 1 backbone with ConL3/ConR4 and Tn7L

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Tags

GFP

Expression

Insect

Mutation

GentR R98K

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMB.Pos6

Plasmid

#234588

Purpose

Moclo Baculo toolkit level 1 backbone with ConL5/ConRE and Tn7L

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Tags

GFP

Expression

Insect

Mutation

GentR R98K

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pM.004

Plasmid

#228690

Purpose

C terminal dual protein A affinity tag with TEV cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

TEV-2xProtein A

Expression

Insect

Mutation

WT

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pM.003

Plasmid

#228689

Purpose

N terminal dual protein A affinity tag with TEV cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

2xProtein A-TEV

Expression

Insect

Mutation

WT

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pM.002

Plasmid

#228688

Purpose

C terminal MBP affinity tag with PreScission Protease cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

Prs-MBP

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pM.001

Plasmid

#228687

Purpose

N terminal MBP affinity tag with PreScission Protease cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

MBP-Prs

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-HIS3

Plasmid

#122908

Purpose

LYS2-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pFH43

Plasmid

#126020

Purpose

Level0 Pea RbSC E9 terminator

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Pea RbSC E9 terminator

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH48

Plasmid

#125813

Purpose

Level0 Csy4

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Csy4

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH40

Plasmid

#125879

Purpose

Level0 Switchgrass PvUBI1 Pol II promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Switchgrass PvUbi1 Pol II promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGGEV_-1_XcmI-LacZ

Plasmid

#49296

Purpose

Golden Gate entry vector at position 1, no 5' ATG, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_-1_FRT_+1_OA-

Plasmid

#49310

Purpose

Golden Gate entry vector: Position 1, FRT site

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Insert

FRT forward

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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