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pDONR223_MAX_p.R35L

Plasmid

#82732

Purpose

Gateway Donor vector containing MAX, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway entry vector

Mutation

R35L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.W450L

Plasmid

#82830

Purpose

Gateway Donor vector containing BRAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

W450L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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p3E mCherry-T2A-hsHRAS-G12V-pA (JDW 1188)

Plasmid

#242571

Purpose

Gateway 3' entry clone containing mCherry followed by a T2A cleavage peptide and then human HRAS G12V.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

HRAS-G12V (HRAS Human)

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME-B-catenin-S33Y-FLAG (JDW 419)

Plasmid

#242565

Purpose

Gateway middle entry clone containing dominant active B-catenin (S33Y beta catenin)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

S33Y B-catenin (CTNNB1 Human)

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME-V5-mTagBFP2-KRAS4A-WT (JDW 831)

Plasmid

#242568

Purpose

Gateway middle entry clone containing an mTagBFP2 fused human KRAS4A WT.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

KRAS4A (WT) (KRAS Human)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-V5-mClover-KRAS4A-WT (JDW 812)

Plasmid

#242566

Purpose

Gateway middle entry clone containing an mClover3 fused human KRAS4A WT.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

KRAS4A (WT) (KRAS Human)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-CNDP2-E166D

Plasmid

#226720

Purpose

Mammalian expression of cytosolic mouse CNDP2 mutant E166D

Depositor

Jonathan Long

Article

Moya-Garzon et al Cell. 2025 Jan 9;188(1):175-186.e20. doi

Insert

CNDP2 (Cndp2 Mouse)

Expression

Mammalian

Mutation

E166D

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-CNDP2-E166L

Plasmid

#226721

Purpose

Mammalian expression of cytosolic mouse CNDP2 mutant E166L

Depositor

Jonathan Long

Article

Moya-Garzon et al Cell. 2025 Jan 9;188(1):175-186.e20. doi

Insert

CNDP2 (Cndp2 Mouse)

Expression

Mammalian

Mutation

E166L

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-CNDP2-D195A

Plasmid

#226722

Purpose

Mammalian expression of cytosolic mouse CNDP2 mutant D195A

Depositor

Jonathan Long

Article

Moya-Garzon et al Cell. 2025 Jan 9;188(1):175-186.e20. doi

Insert

CNDP2 (Cndp2 Mouse)

Expression

Mammalian

Mutation

D195A

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6-CNDP2-H445F

Plasmid

#226723

Purpose

Mammalian expression of cytosolic mouse CNDP2 mutant H445F

Depositor

Jonathan Long

Article

Moya-Garzon et al Cell. 2025 Jan 9;188(1):175-186.e20. doi

Insert

CNDP2 (Cndp2 Mouse)

Expression

Mammalian

Mutation

H445F

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-SNAP-p53DD-IRES-HRAS(G12V)

Plasmid

#233201

Purpose

Gateway entry vector for SNAP-p53DD and HRAS(G12V)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

SNAP-p53DD-IRES-HRAS(G12V) (Trp53, HRAS Mouse, Human)

Use

Other

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-HRAS(G12V)-IRES-SNAP-p53DD

Plasmid

#233199

Purpose

Gateway entry vector for HRAS(G12V) and SNAP-p53DD

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-SNAP-p53DD (HRAS, Trp53 Mouse, Human)

Use

Other

Mutation

G12V

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-HRAS(G12V)-IRES-p53DD

Plasmid

#233186

Purpose

Gateway entry vector for HRAS(G12V) and p53DD (RIDD)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-p53DD (HRAS, Trp53 Mouse, Human)

Use

Other

Mutation

G12V

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-p53DD-IRES-HRAS(G12V)

Plasmid

#233188

Purpose

Gateway entry vector for p53DD and HRAS(G12V) (DDIR)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

p53DD-IRES-HRAS(G12V) (Trp53, HRAS Mouse, Human)

Use

Other

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227925

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

YWHAQ homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227926

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

YWHAB homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML600_COG8-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227911

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

COG8 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML357_CAV1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227914

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CAV1 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML361_DCP1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227918

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

DCP1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML366_HSPA1B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227920

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

HSPA1B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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