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Showing: 901 - 920 of 8661 results

  1. pJK611

    Plasmid
    #72532
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72532-plasmid-map-sequence-id-132620

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  2. pJK626

    Plasmid
    #72461
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to cysteine, tyrosine-…
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72461-plasmid-map-sequence-id-133899

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  3. pJK691

    Plasmid
    #72381
    Purpose
    Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)
    Depositor
    T. J. Kappock
    Insert
    stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72381-plasmid-map-sequence-id-134006

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  4. pJK292

    Plasmid
    #73371
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/His89>Asn mutant (AaPurE1-H59N,H89N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed histidine-59 to asparagine and histidine-…
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73371-plasmid-map-sequence-id-135107

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  5. pJK293

    Plasmid
    #73372
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Phe/His89>Phe mutant (AaPurE1-H59F,H89F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed histidine-59 to phenylalanine and histidi…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73372-plasmid-map-sequence-id-135108

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  6. pJK294

    Plasmid
    #73373
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed histidine-59 to aspartate and histidine-8…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73373-plasmid-map-sequence-id-135109

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  7. pJK528

    Plasmid
    #71625
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met105>Ser/Ile109>Trp mutant (TdPurE2-M105S,I109W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes methionine-105 to serine, isoleucine-109 …
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71625-plasmid-map-sequence-id-131668

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  8. pJK439

    Plasmid
    #72478
    Purpose
    Produces Thermoplasma acidophilum citrate synthase pseudo-dimer
    Depositor
    T. J. Kappock
    Insert
    citrate synthase (artificial dimer)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted NSTCLVPRGSPV sequence between C- and N-t…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72478-plasmid-map-sequence-id-134617

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  9. pJK404

    Plasmid
    #72417
    Purpose
    Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    additional N-terminal 15 residues MSETYGTGQKEQHC,…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72417-plasmid-map-sequence-id-135656

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  10. pJK268

    Plasmid
    #72436
    Purpose
    Produces Acetobacter aceti 1023 del7-GloA
    Depositor
    T. J. Kappock
    Insert
    lactoylglutathione lyase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Missing residues 1-7
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72436-plasmid-map-sequence-id-132660

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  11. pJK627

    Plasmid
    #72462
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp/Tyr154>Phe mutant (AaPurE1-S57D,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to aspartate, tyrosine…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72462-plasmid-map-sequence-id-133901

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  12. pJK628

    Plasmid
    #72463
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Ala/Tyr154>Phe mutant (AaPurE1-S57A,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to alanine, tyrosine-1…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72463-plasmid-map-sequence-id-133902

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  13. pJK573

    Plasmid
    #72456
    Purpose
    Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~57 silent substitutions
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72456-plasmid-map-sequence-id-133910

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  14. pJK632

    Plasmid
    #72465
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val/Tyr154>Phe mutant (AaPurE1-S57V,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to valine, tyrosine-15…
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72465-plasmid-map-sequence-id-133904

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  15. pJK629

    Plasmid
    #72464
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr/Tyr154>Phe mutant (AaPurE1-S57T,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to threonine, tyrosine…
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72464-plasmid-map-sequence-id-133903

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  16. pJK541

    Plasmid
    #72447
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72447-plasmid-map-sequence-id-133869

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  17. pJK569

    Plasmid
    #72455
    Purpose
    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~51 silent substitutions
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72455-plasmid-map-sequence-id-133906

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  18. pJK587

    Plasmid
    #71696
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag and Cys138>Ser mutant (AaTrxB1H6-C138S)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase 1
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes cysteine-138 to serine
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71696-plasmid-map-sequence-id-131733

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  19. pJK525

    Plasmid
    #71601
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp97>Tyr mutant (AaPurE1fyw)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tryptopha…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71601-plasmid-map-sequence-id-131675

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  20. pJK526

    Plasmid
    #71602
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-97 to tyrosine, tryptophan-165…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71602-plasmid-map-sequence-id-131676

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Showing: 901 - 920 of 8661 results