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Plasmid#12745PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf57
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
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GST-SEPT5 WT
Plasmid#27269DepositorInsertSeptin 5 (SEPTIN5 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
SEPT5 Mut(S161A/S327A)
Plasmid#27275DepositorInsertSeptin 5 (SEPTIN5 Human)
UseTagsMyc (MASMQKLISEEDL)ExpressionMammalianMutationS161A and S327APromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
SEPT5 Mut(S327A)
Plasmid#27274DepositorInsertSeptin 5 (SEPTIN5 Human)
UseTagsMyc (MASMQKLISEEDL)ExpressionMammalianMutationS327APromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
GST-SEPT5 S327A
Plasmid#27271DepositorInsertSeptin 5 (SEPTIN5 Human)
UseTagsGSTExpressionBacterialMutationS327APromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
PME-1 pcDNA-StrepIII
Plasmid#119292PurposeMammalian overexpression of StrepII-tagged full length PPME1DepositorInsertPPME1 (PPME1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
PME-1_R369D mutant
Plasmid#119294PurposeMammalian overexpression of StrepII-tagged full length PPME1 with mutation in the PP2Ac binding motifDepositorInsertPPME1 (PPME1 Human)
UseTagsExpressionMammalianMutationR369DPromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-R-GECO1
Plasmid#32444PurposeMammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorInsertR-GECO1.0
UseTagsExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7d-5p
Plasmid#103151PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7d-5p target (MIRLET7D Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-155-5p
Plasmid#103265PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-155-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-155-5p target (MIR155 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-NLS-R-GECO
Plasmid#32462PurposeMammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorInsertR-GECO1.0
UseTagsNLS sequence DPKKKRKVExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-19a-3p
Plasmid#103323PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-19a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-19a-3p target (MIR19A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-ER-LAR-GECO1
Plasmid#61244PurposeExpresses LAR-GECO1 in the endoplasmic reticulum in mammalian cellsDepositorInsertLAR-GECO1
UseTagsER-retention sequence: KDEL and ER-targeting sequ…ExpressionMammalianMutationSubstitutions relative to R-GECO1: V51W/I113V/N35…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7b-5p
Plasmid#103148PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7b-5p target (MIRLET7B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-LAR-GECO1.2
Plasmid#61245PurposeExpresses LAR-GECO1.2 in the mitochondria in mammalian cellsDepositorInsertLAR-GECO1.2
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationSubstitutions relative to R-GECO1.2: N45I/A47R/E1…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-548e-5p
Plasmid#103634PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-548e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-548e-5p target (MIR548E Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
APEX2 - Actin in pEGFP
Plasmid#66172PurposeAPEX2 fused to the N-terminus of human beta actinDepositorInsertAPEX2 - human beta Actin (ACTB Human, Synthetic)
UseTagsFlag epitope tag (DYKDDDDK)ExpressionMammalianMutationAPEX2 contains 4 mutations relative to wt soybean…PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-R-GECO1
Plasmid#32465DepositorInsertR-GECO1.0
UseLab constructedTags6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…ExpressionBacterialMutationSubstitutions relative to the mApple-derived anal…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMV-REX-GECO1
Plasmid#61246PurposeExpresses REX-GECO1 in mammalian cellsDepositorInsertREX-GECO1
UseTagsExpressionMammalianMutationSubstitutions relative to R-GECO1: P60R, V61W, R6…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only