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p3A2-HA

Plasmid

#234804

Purpose

Mammalian expression of bovine arrestin-2 with C-terminal HA tag

Depositor

Vsevolod Gurevich

Article

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

Arrestin-2 (ARRB2 Bovine)

Tags

HA

Expression

Mammalian

Mutation

none

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3b_031_mVenus

Plasmid

#235884

Purpose

mVenus N-tag

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

mVenus N-tag

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5a_008_mCerulean

Plasmid

#235890

Purpose

mCerulean C-tag

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

mCerulean C-tag

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5a_009_mVenus

Plasmid

#235891

Purpose

mVenus C-tag

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

mVenus C-tag

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3b_029_mScarlet-I

Plasmid

#235882

Purpose

mScarlet-I N-tag

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

mScarlet-I N-tag

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3b_030_mCerulean

Plasmid

#235883

Purpose

mCerulean N-tag

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

mCerulean N-tag

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-pri-miR-Ctrl

Plasmid

#235158

Purpose

Expresses EGFP and a control primary transcript in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/primary transcript with control siRNA

Use

AAV

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-miR-206-sponge

Plasmid

#235154

Purpose

Expresses a EGFP-based sponge for miR-206 in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/miR-206 sponge with 12x binding sites

Use

AAV

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-miR-133-sponge

Plasmid

#235155

Purpose

Expresses a EGFP-based sponge for miR-133 in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/miR-133 sponge with 12x binding sites

Use

AAV

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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p3A3-HA

Plasmid

#234806

Purpose

Mammalian expression of bovine arrestin-3 with C-terminal HA tag

Depositor

Vsevolod Gurevich

Article

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

Arrestin-3 (ARR3 Bovine)

Tags

HA

Expression

Mammalian

Mutation

none

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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p3A2-Flag

Plasmid

#234803

Purpose

Mammalian expression of bovine arrestin-2 with C-terminal Flag tag

Depositor

Vsevolod Gurevich

Article

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

Arrestin-2 (ARRB2 Bovine)

Tags

Flag

Expression

Mammalian

Mutation

none

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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P1909:EVA4_RHISA-StrepII-8xHis

Plasmid

#233698

Purpose

Expresses Strep and His-tagged Evasin 4 (EVA4_RHISA) in mammalian cells

Depositor

Shoumo Bhattacharya

Article

Kryukova et al Commun Biol. 2025 Apr 3;8(1):549. doi: 1

Insert

EVA4

Tags

Strep II, 8xHis

Expression

Mammalian

Promoter

Chicken β actin

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-TEV-AtPCO4-2(wt)

Plasmid

#191802

Purpose

Full length wt AtPCO4-2 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO4-2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-pfv-Sapphire

Plasmid

#231957

Purpose

The plasmid containing TRE-pfv-Sapphire for the doxycycline-inducible expression of genetically encoded multimeric nanoparticles (GEM)

Depositor

Michael Shtutman

Article

Korunova et al bioRxiv [Preprint]. 2024 Nov 18:2024.11.

Insert

genetically encoded multimeric nanoparticles

Use

Lentiviral; Doxocycline inducible

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMS19

Plasmid

#229475

Purpose

Expresses RssB with E135A, E136A, & E137A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS21

Plasmid

#229476

Purpose

Expresses RssB with E135A, E136A, & E137A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Tags

10xHis

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF18

Plasmid

#229456

Purpose

Expresses IraD (aa17-130) with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (aa 17-130 only)

Tags

6xHis

Expression

Bacterial

Mutation

aa 17-130 only

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(1-573)

Plasmid

#219683

Purpose

Mammalian expression of CLIPf-KIF5A(1-573)

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf

Expression

Mammalian

Mutation

1-573

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A

Plasmid

#200790

Purpose

Mammalian expression of CLIPf-KIF5A

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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p8270 LentiCRISPR v2 hygro sgSIGIRR-3

Plasmid

#193979

Purpose

Expression of spCas9 and sgRNA targeting SIGIRR

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)

Use

Lentiviral

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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